Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources
Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources
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DOI：
10.1139/g07-007
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发表时间：
2007-03-01
期刊：
影响因子：
3.1
通讯作者：
Carneiro Vieira, Maria Lucia
中科院分区：
文献类型：
--
作者：
Hanai, Luiz Ricardo;de Campos, Tatiana;Carneiro Vieira, Maria Lucia
微卫星或SSR(单序列重复)已被用于构建和整合包括菜豆在内的作物物种的遗传图谱。在本研究中，对由Bean EST计划(http://1gm.esalq.usp.br/BEST/)，)构建的3个cDNA库进行了分析，其中包括3126个序列的Unigene集合和一个基因组微卫星丰富的文库。共有219个表达序列标签(EST)携带240个SSR(命名为EST-SSR)，而714个基因组序列包含471个SSR(命名为基因组SSR)。对来自中美洲和安第斯山脉基因库的23个栽培豆品种(包括巴西品种和2个近缘种)的80个SSR、40个EST-SSR和40个基因组SSR进行了分子多态性分析。在普通菜豆品种中，31个EST-SSR基因座为多态，产生2-12个等位基因，26个基因组SSR为多态，共产生2-7个等位基因。使用基因和基因组SSR的数据进行的聚类分析表明，安第斯和中美洲豆类之间存在明显的分离。讨论了这些基因座在区分菜豆基因类型和遗传作图中的作用。
Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. ln the present study, 3 cDNA libraries generated by the Bean EST project (http://1gm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genomic microsatellite-enriched library, were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR). A subset of 80 SSRs, 40 EST-SSRs, and 40 genomic-SSRs were evaluated for molecular polymorphism in 23 genotypes of cultivated beans from the Mesoamerican and Andean genetic pools, including Brazilian cultivars and 2 related species. Of the common bean genotypes, 31 EST-SSR loci were polymorphic, yielding 2-12 alleles as compared with 26 polymorphic genomic-SSRs, accounting for 2-7 alleles. Cluster analysis from data using both genic and genomic-SSR revealed a clear separation between Andean and Mesoamerican beans. The usefulness of these loci for distinguishing bean genotypes and genetic mapping is discussed.