Integrated transcriptomic and proteomic characterization of a chromosome segment substitution line reveals a new regulatory network controlling the seed storage profile of soybean
Integrated transcriptomic and proteomic characterization of a chromosome segment substitution line reveals a new regulatory network controlling the seed storage profile of soybean
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DOI：
10.1002/fes3.381
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发表时间：
2022-03
影响因子：
5
通讯作者：
Xue Han;Jiapeng Li;Yabin Zhao;Zhanguo Zhang;Hongwei Jiang;Jinxing Wang;Xuezhen Feng;Yu Zhang-Yu-Zh
中科院分区：
文献类型：
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作者：
Xue Han;Jiapeng Li;Yabin Zhao;Zhanguo Zhang;Hongwei Jiang;Jinxing Wang;Xuezhen Feng;Yu Zhang-Yu-Zh
大豆是世界上提供油和蛋白质的主要作物。几个世纪以来，大豆制品在许多不同种类的食物中被消费。控制种子中脂肪酸（FA）和贮藏蛋白（SSP）积累的调控网络知之甚少。为了获得对高质量种子的分子机制的新见解，分析了种子FA和SSP含量对野生大豆品系（CSSL）中染色体片段替换的影响。对CSSL和亲本系的干种子进行了高通量转录组学和基于串联质量标签（TMT）的定量蛋白质组学研究。共鉴定出665个差异表达基因（DEG）和83个差异积累蛋白（DAP）。其中27个DEG和23个DAP被发现调节种子储存概况。这些基因编码参与光合作用、蛋白质加工、蛋白质分选和储存蛋白质积累的蛋白质。所提供的数据显示FA合成通过SSP积累相关基因和蛋白的调节而减少。两者合计，结果提供了新的见解，涉及种子储存化合物的积累，涉及源和库的过程，可用作标记，在未来的大豆育种计划的调节网络。
Soybean is a major crop that provides oil and protein worldwide. Soybean products have been consumed for centuries in many different kinds of food. The regulatory network that controls the accumulation of fatty acids (FAs) and storage proteins (SSPs) in seeds is poorly understood. To gain new insights into the molecular mechanisms that contribute to high‐quality seeds, seed FA and SSP contents were analyzed for the effects of chromosome segment substitution in a wild soybean line (CSSL). High‐throughput transcriptomics and tandem mass tag (TMT)‐based quantitative proteomics were performed on the dry seeds of the CSSL and parent lines. In total, 665 differentially expressed genes (DEGs) and 83 differentially accumulated proteins (DAPs) were identified. Of these 27 DEGs and 23 DAPs were found to regulate the seed storage profile. These genes encode proteins involved in photosynthesis, protein processing, protein sorting, and storage protein accumulation. Data are presented showing that FA synthesis was decreased by the regulation of SSP‐accumulation‐related genes and proteins. Taken together, the results provide new insights into the regulation network involved in the accumulation of seed storage compounds involving both source and sink processes that may be used as markers in future soybean breeding programs.