Natural killer cell enumeration and function in HIV-infected and high-risk uninfected adolescents
Natural killer cell enumeration and function in HIV-infected and high-risk uninfected adolescents
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DOI：
10.1089/08892220151126643
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发表时间：
2001-04-10
影响因子：
1.5
通讯作者：
Wilson, C
中科院分区：
文献类型：
--
作者：
Douglas, SD;Durako, SJ;Wilson, C
这是第一次报告自然杀伤细胞计数和功能在艾滋病毒感染和高风险未感染的青少年。我们研究了该队列的人口统计学特征与三个结果的相关性：CD 16（+）细胞绝对计数，每个外周血单核细胞（PBMC）的溶解单位和每个自然杀伤（NK）细胞的溶解单位。我们还研究了CD 4、CD 38和抗逆转录病毒治疗（ART）的使用与HIV感染青少年亚组中这些结果的相关性。对参与正在进行的纵向研究（REACH研究）的青少年进行CD 16（+）细胞计数和NK功能采样。对412例有NK细胞数据的受试者进行了横断面分析。HIV阳性男性的CD 3（-）/CD 16（+）/CD 56（+）NK细胞数量高于HIV阳性女性，但是，对于HIV阴性受试者，我们没有观察到NK细胞绝对数量的性别相关效应。然而，性别是分析的显著协变量，使用每个PBMC的裂解单位作为测量单位，男性显示出高于女性的值。对于检查的NK细胞数量和功能的三项评估中的任何一项，年龄都不是预测协变量。我们对HIV阳性个体的观察表明，CD 4（+）T细胞计数减少与循环中CD 3（-）/CD 16（+）/CD 56（+）NK细胞减少有关。我们还观察到CD 8（+）/CD 38（+）/DR+淋巴细胞的升高与每个PBMC的NK细胞溶解单位的降低之间存在相关性。我们的多变量模型的结果表明，在接受单药或多药抗逆转录病毒治疗的患者中，NK细胞数量减少，每个PBMC的裂解单位减少。与高危HIV阴性青少年相比，HIV感染青少年的循环NK细胞数量和功能发生了变化。这些数据表明，这些变化可能发生在HIV病程的早期，但随着CD 4（+）T细胞库的消耗，数量的变化继续发生。
This is the first report of natural killer cell enumeration and function in HIV-infected and high-risk uninfected adolescents. We examined the association of demographic characteristics of this cohort with three outcomes: CD16(+) cell absolute count, lytic units per peripheral blood mononuclear cell (PBMC), and lytic units per natural killer (NK) cell. We also examined the association of CD4, CD38, and antiretroviral therapy (ART) use with these outcomes in the subset of HIV-infected adolescents. Adolescents participating in an on-going longitudinal study (the REACH study) were sampled for CD16(+) cell count and NK function. This cross-sectional analysis was performed on 412 subjects with NK cell data available. HIV-positive males had higher numbers of CD3(-)/CD16(+)/CD56(+) NK cells than HIV-positive females, However, for the HIV-negative subjects, we did not observe a gender-related effect for absolute NK cell numbers. Gender, however, was a significant covariate for the analysis, using lytic units per PBMC as the unit of measurement, with males showing higher values than females. Age was not a predictive covariate for any of the three assessments of NK cell number and function examined. Our observations concerning the HIV-positive individuals indicate that reduced CD4(+) T cell counts were associated with decreased circulating CD3(-)/CD16(+)/CD56(+) NK cells. We also observed an association between elevation of CD8(+)/CD38(+)/DR+ lymphocytes and lower NK lytic units per PBMC. The results of our multivariate models indicate that there is a reduced number of NK cells and reduced lytic units per PBMC in patients receiving single or multidrug antiretroviral therapy. There are changes in circulating NK cell number and function in HIV-infected adolescents, in comparison with high-risk HIV-negative adolescents. The data suggest that these changes may occur early in the course of HIV disease but that quantitative changes continue to occur with advancing depletion of the CD4(+) T cell pool.