Comparison of the synergistic effect of lipid nanobubbles and SonoVue microbubbles for high intensity focused ultrasound thermal ablation of tumors.
Comparison of the synergistic effect of lipid nanobubbles and SonoVue microbubbles for high intensity focused ultrasound thermal ablation of tumors.
复制标题
脂质纳米泡与SonoVue微泡高强度聚焦超声热消融肿瘤的协同效应比较
作者：
Yao Y;Yang K;Cao Y;Zhou X;Xu J;Liu J;Wang Q;Wang Z;Wang D
微泡被认为是高强度聚焦超声（HIFU）治疗良性或恶性肿瘤的重要增强剂。最近，已经研究了不同尺寸的充气气泡以提高HIFU热消融的治疗效率并减少与超声功率和照射时间相关的副作用。然而，纳米气泡（NB）作为超声造影剂的协同治疗HIFU热消融仍然存在争议，由于其直径小的纳米尺寸。在这项研究中，磷脂壳和气核NBs的窄尺寸范围为500 - 600 nm的开发。在离体牛肝和兔乳腺肿瘤模型中仔细研究了NBs对HIFU热消融的协同作用，并与商业SonoVue微泡（SonoVue MBs）进行了关键性比较。进一步观察肿瘤组织靶区在HIFU消融后的病理变化。使用磷酸盐缓冲盐水（PBS）作为对照。在相同的HIFU参数下，脂质NBs组的B超图像定量回声强度和凝固性坏死体积明显高于PBS组，但与SonoVue MBs组相比无显著性差异。这些结果表明，脂质NBs对HIFU热消融的协同作用与SonoVue MBs相似，并进一步表明脂质NBs可能成为HIFU热消融肿瘤的增强剂。
Microbubbles (MBs) are considered as an important enhancer for high intensity focused ultrasound (HIFU) treatment of benign or malignant tumors. Recently, different sizes of gas-filled bubbles have been investigated to improve the therapeutic efficiency of HIFU thermal ablation and reduce side effects associated with ultrasound power and irradiation time. However, nanobubbles (NBs) as an ultrasound contrast agent for synergistic therapy of HIFU thermal ablation remain controversial due to their small nano-size in diameter. In this study, phospholipid-shell and gas-core NBs with a narrow size range of 500–600 nm were developed. The synergistic effect of NBs for HIFU thermal ablation was carefully studied both in excised bovine livers and in breast tumor models of rabbits, and made a critical comparison with that of commercial SonoVue microbubbles (SonoVue MBs). In addition, the pathological changes of the targeted area in tumor tissue after HIFU ablation were further investigated. Phosphate buffer saline (PBS) was used as the control. Under the same HIFU parameters, the quantitative echo intensity of B-mode ultrasound image and the volume of coagulative necrosis in lipid NBs groups were significantly higher and larger than that in PBS groups, but could not be demonstrated a difference to that in SonoVue MBs groups both ex vivo and in vivo. These results showed that the synergistic effect of lipid NBs for HIFU thermal ablation were similar with that of SonoVue MBs, and further indicate that lipid NBs could potentially become an enhancer for HIFU thermal ablation of tumors.