COMPLETE NUCLEOTIDE-SEQUENCE OF THE ATTENUATED POLIOVIRUS SABIN-1 STRAIN GENOME
COMPLETE NUCLEOTIDE-SEQUENCE OF THE ATTENUATED POLIOVIRUS SABIN-1 STRAIN GENOME
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DOI：
10.1073/pnas.79.19.5793
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
IMURA, N
中科院分区：
文献类型：
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作者：
NOMOTO, A;OMATA, T;IMURA, N
采用分子克隆和快速序列分析技术确定了1型脊髓灰质炎病毒疫苗株（LSc，2ab）基因组的完整核苷酸序列。将由疫苗株RNA合成的双链c[互补]DNA的限制性片段插入克隆载体pBR322的适当位点。克隆 DNA 的序列分析显示，病毒体 RNA 分子长 7441 个核苷酸，并在 3'' 末端多聚腺苷酸化。当将核苷酸序列与强毒亲本菌株（Mahoney）的基因组的核苷酸序列进行比较时，观察到57个碱基取代分散在整个基因组中。其中，21 种导致了许多病毒蛋白的氨基酸变化。一组氨基酸变化位于病毒外壳蛋白中，尤其是病毒衣壳蛋白 VP1 的 NH2 末端部分。因此，VP1 编码区的突变显然有助于减弱。
The complete nucleotide sequence of the genome of the type 1 poliovirus vaccine strain (LSc,2ab) was determined by using molecular cloning and rapid sequence analysis techniques. The restriction fragments of double-stranded c[complementary]DNA synthesized from the vaccine strain RNA were inserted into the adequate sites of cloning vector pBR322. Sequence analysis of the cloned DNA revealed that the virion RNA molecule was 7441 nucleotides long and polyadenylylated at the 3'' terminus. When the nucleotide sequence was compared with that of the genome of the virulent parental strain (Mahoney), 57 base substitutions were observed to be scattered all over the genome. Of these, 21 resulted in amino acid changes in a number of viral proteins. A cluster of amino acid changes is located in the viral coat proteins, especially in the NH2-terminal half of the viral capsid protein VP1. Thus, the mutations in the VP1 coding region evidently contribute to attenuation.