Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens.
Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens.
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H-2L 分子的一级结构研究证实，它是与 H-2K 和 H-2D 抗原具有同源性的独特基因产物。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
T. Hansen
中科院分区：
文献类型：
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作者：
J. Coligan;T. Kindt;R. Nairn;S. Nathenson;D. Sachs;T. Hansen
放射化学方法已被用于小鼠H-2Ld主要组织相容性复合体基因产物的分离和初步生化表征。通过免疫沉淀从洗涤剂溶解的H-2d肿瘤细胞的糖蛋白部分分离放射性标记的分子。从该分子的木瓜蛋白酶片段中分离出六个主要的CNBr片段;其中三个片段通过二硫键连接。由于主要移植抗原之间的高度同源性，通过比较它们的氨基酸序列与H-2Kb基因产物的氨基酸序列来比对片段是可能的。在可用于H-2Ld和H-2Kb、H-2Dd和H-2Kd基因产物之间比较的位置中，80个中的61个（78%）、55个中的45个（82%）和15个中的12个（80%）分别是相同的。Ld和Kb和Dd分子之间的差异分布在整个氨基酸序列中。这些数据表明，H-2Ld基因产物是不同于H-2K和H-2D基因产物的分子种类，但与H-2K和H-2D基因产物同源。
Radiochemical methodology has been used in the isolation and preliminary biochemical characterization of the murine H-2Ld major histocompatibility complex gene product. The radiolabeled molecule was isolated by immunoprecipitation from the glycoprotein fraction of detergent-solubilized H-2d tumor cells. Six major CNBr fragments were isolated from a papain fragment of this molecule; three of the fragments are connected by disulfide bonds. Due to the high degree of homology between major transplantation antigens, it was possible to align the fragments by comparison of their amino acid sequences to that of the H-2Kb gene product. Of the positions available for comparison between H-2Ld and H-2Kb, H-2Dd, and H-2Kd gene products, 61 out of 80 (78%), 45 out of 55 (82%), and 12 out of 15 (80%), respectively, are identical. Differences between the Ld and Kb and Dd molecules are distributed throughout the amino acid sequence. These data indicate that the H-2Ld gene product is a molecular species distinct from, but homologous to, the H-2K and H-2D gene products.
鼠主要组织相容性复合物同种抗原的一级结构：完成 H-2Kb 氨基末端 284 个残基的序列。
影响因子：
2.9
作者：
Martinko,JM;Uehara,H;Ewenstein,BM;Kindt,TJ;Coligan,JE;Nathenson,SG
通讯作者：
Nathenson,SG