Age-associated thymic atrophy is not associated with a deficiency in the CD44+CD25-CD3-CD4-CD8- thymocyte population
Age-associated thymic atrophy is not associated with a deficiency in the CD44+CD25-CD3-CD4-CD8- thymocyte population
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DOI：
10.1006/cimm.2001.1848
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发表时间：
2001-09-15
影响因子：
4.3
通讯作者：
Andrew, D
中科院分区：
文献类型：
--
作者：
Aspinall, R;Andrew, D
年龄相关的胸腺萎缩被认为是由于胸腺微环境的改变和早期T细胞祖细胞CD44(+)CD25(-)CD3(-)CD4(-)CD8（-）细胞的内在特性的改变。我们已经从老年和年轻小鼠的胸腺中纯化了这些细胞，并证明当用于重建胎儿胸腺器官培养时，它们在体外分化为后代的能力没有年龄相关的缺陷。我们还证明，与对照组相比，在抗il -7的存在下，来自年轻小鼠的CD44(+)CD25(-)CD3(-)CD4(-)CD8（-）细胞在胎儿胸腺器官培养中显示出胸腺细胞发育减少。最后，我们已经表明，与对照组相比，用IL-7治疗的老年小鼠胸腺功能得到改善。在老年动物中观察到的增加的胸腺功能以顺序的方式发生，这是预期的药物直接作用于早期阶段，包括CD44(+)CD25(-)CD3(-)CD4(-)CD8（-）细胞。(C) 2001 Elsevier Science。
Age-associated thymic atrophy has been proposed to be due to changes in both the thymic microenviromnent and in the intrinsic properties of the early T cell progenitors, the CD44(+)CD25(-)CD3(-)CD4(-)CD8(-) cells. We have purified these cells from the thymus of both old and young mice and demonstrate no age-associated defect in their ability to differentiate into their progeny in vitro when used to reconstitute fetal thymic organ cultures. We also demonstrate that in the presence of anti-IL-7, CD44(+)CD25(-)CD3(-)CD4(-)CD8(-) cells from young mice show reduced thymocyte development in fetal thymic organ cultures compared with controls. Finally we have shown that old mice treated with IL-7 show improved thymopoiesis compared with control groups. The increased thymopoiesis seen in the old animals occurs in the sequential manner which would be anticipated for an agent working directly on the early stages, including the CD44(+)CD25(-)CD3(-)CD4(-)CD8(-) cells. (C) 2001 Elsevier Science.