Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34(+) cells
Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34(+) cells
复制标题
双网络水凝胶中干细胞因子的持续释放用于脐带血来源的 CD34( ) 细胞的离体培养
DOI：
10.1111/cpr.12407
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发表时间：
2018
影响因子：
8.5
通讯作者：
Weian Zhang
中科院分区：
文献类型：
--
作者：
Yuanhao Zhang;Xiuwei Pan;Zhen Shi;Haibo Cai;Yun Gao;Weian Zhang
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ObjectivesStem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.Materials and methodsStem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydrogel to achieve a slow release rate. CD34+cells were cultured within the SCF‐loaded HGDN hydrogel for 14 days. The cell number, phenotype and functional capacity were investigated after culture.ResultsThe HGDN hydrogels had desirable properties and encapsulated SCF kept being released for more than 6 days. SCF remained the native bioactivity, and the proliferation of HSCs within the SCF‐loaded HGDN hydrogel was not affected, although the consumption of SCF was only a quarter in comparison with the conventional culture. Moreover, CD34+cells harvested from the SCF‐loaded HGDN hydrogels generated more multipotent colony‐forming units (CFU‐GEMM).ConclusionThe data suggested that the SCF‐loaded HGDN hydrogel could support ex vivo culture of HSCs, thus providing a cost‐effective culture protocol for HSCs.