High-resolution imaging of the osteogenic and angiogenic interface at the site of murine cranial bone defect repair via multiphoton microscopy.
High-resolution imaging of the osteogenic and angiogenic interface at the site of murine cranial bone defect repair via multiphoton microscopy.
复制标题
通过多光子显微镜对小鼠颅骨缺损修复部位的成骨和血管生成界面进行高分辨率成像。
DOI：
10.7554/elife.83146
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发表时间：
2022-11-03
期刊：
影响因子：
7.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Schilling K;Zhai Y;Zhou Z;Zhou B;Brown E;Zhang X
利用高分辨率多光子成像平台，结合先进的光学技术，研究了小鼠颅骨骨缺损修复过程中成骨和血管生成界面的时空血管形成和规范，该平台可以探测活体动物的氧微环境和细胞能量代谢。我们的研究显示颅骨骨缺损上、硬膜骨膜血管类型的动态变化，即动脉、静脉和毛细血管网络，提示修复过程中血管类型与成骨细胞扩张和骨组织沉积/重塑之间存在差异耦合。利用转基因报告小鼠模型标记修复部位不同类型的血管，我们进一步表明，愈合部位毛细血管中的氧气分布是不均匀的，并且依赖于时间和位置。与成骨细胞偶联的内皮细胞更倾向于糖酵解，对微环境氧变化的敏感性低于成骨细胞。相比之下，成骨细胞利用相对较多的OxPhos，并可能在修复部位消耗更多的氧气。综上所述，我们的研究强调了缺陷修复部位血管类型的动力学和功能意义，为进一步描述骨组织再生界面的氧气和代谢微环境开辟了机会。
The spatiotemporal blood vessel formation and specification at the osteogenic and angiogenic interface of murine cranial bone defect repair were examined utilizing a high-resolution multiphoton-based imaging platform in conjunction with advanced optical techniques that allow interrogation of the oxygen microenvironment and cellular energy metabolism in living animals. Our study demonstrates the dynamic changes of vessel types, that is, arterial, venous, and capillary vessel networks at the superior and dura periosteum of cranial bone defect, suggesting a differential coupling of the vessel type with osteoblast expansion and bone tissue deposition/remodeling during repair. Employing transgenic reporter mouse models that label distinct types of vessels at the site of repair, we further show that oxygen distributions in capillary vessels at the healing site are heterogeneous as well as time- and location-dependent. The endothelial cells coupling to osteoblasts prefer glycolysis and are less sensitive to microenvironmental oxygen changes than osteoblasts. In comparison, osteoblasts utilize relatively more OxPhos and potentially consume more oxygen at the site of repair. Taken together, our study highlights the dynamics and functional significance of blood vessel types at the site of defect repair, opening up opportunities for further delineating the oxygen and metabolic microenvironment at the interface of bone tissue regeneration.
DOI：
10.1007/978-1-4939-8697-2_17
发表时间：
2018
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Zhang X
通讯作者：
Zhang X