Transcription factor snail1 expression and poor survival in pharyngeal squamous cell carcinoma
Transcription factor snail1 expression and poor survival in pharyngeal squamous cell carcinoma
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DOI：
10.14670/hh-26.443
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发表时间：
2011-04-01
影响因子：
2
通讯作者：
Kosma, Veli-Matti
中科院分区：
文献类型：
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作者：
Jouppila-Matto, Anna;Tuhkanen, Hanna;Kosma, Veli-Matti
Snail 1是上皮-间质转化（EMT）的关键调节因子，在肿瘤进展中起着重要作用。以前对snail的研究主要集中在上皮肿瘤细胞上。本研究旨在探讨Snail 1蛋白在咽部鳞状细胞癌（PSCC）组织中的表达及其与临床病理特征和生存期的关系。使用免疫组织化学分析了110个组织微阵列样本的snail 1表达。在内皮细胞中，107例病例中有51例（48%）观察到snail 1表达，并预测疾病特异性生存期（DSS）降低（p= 0.009）。在49例（46%）肿瘤样本中，在基质肌成纤维细胞中检测到snail 1免疫染色，该组中DSS有变差的趋势（p= 0.067）。内皮细胞和间质肌成纤维细胞中的Snail 1表达也与下咽肿瘤（分别为p= 0.01和p= 0.038）、增加的T分类（T3-4）（分别为p= 0.005，p= 0.037）和患者较差的一般状况（Karnofsky体力状态评分< 70;分别为p= 0.029，p= 0.039）相关。此外，内皮表达与晚期（III-IV）（p= 0.005）和较差分化（2-3级; p= 0.012）相关。在恶性上皮细胞中，75/110例（68%）检测到snail 1免疫染色。蛋白质的表达在下咽肿瘤中更常见（p= 0.044）。Snail 1阳性肿瘤与较低的Karnofsky体能状态评分（p= 0.039）和区域失败（p= 0.042）相关。我们的研究结果表明，snail 1蛋白在内皮细胞中的表达，并在一定程度上也在肿瘤间质肌成纤维细胞似乎是一个预测PSCC的生存不良。在肿瘤微环境中而不是在恶性上皮肿瘤细胞中存在snail 1蛋白可能诱导组织重塑和肿瘤进展。
Snail1, a key regulator of epithelial-mesenchymal transition (EMT), plays an important role in tumour progression. Previous studies of snail1 have mainly focused on the epithelial tumour cells. The objective of this study was to evaluate the expression of snail1 protein in endothelial cells, stromal myofibroblasts and malignant epithelial cells of pharyngeal squamous cell carcinomas (PSCC), as well as its relation to clinicopathological features and survival. One hundred and ten tissue microarray samples were analyzed for snail1 expression using immunohistochemistry. In endothelial cells snail1 expression was observed in 51 (48%) of 107 cases and it predicted reduced disease specific survival (DSS) (p= 0.009). In 49 (46%) tumour samples snail1 immunostaining was detected in stromal myofibroblasts and there was a tendency to poorer DSS in that group (p= 0.067). Snail1 expression in endothelial cells and stromal myofibroblasts is also associated with hypopharyngeal tumours (p= 0.01 and p= 0.038 respectively), increasing T category (T3-4) (p= 0.005, p= 0.037 respectively) and poorer general condition of the patient (Karnofsky performance status score < 70; p= 0.029, p= 0.039 respectively). Moreover endothelial expression correlated with advanced stage (III-IV) (p= 0.005) and poorer differentiation (grade 2-3; p= 0.012). In malignant epithelial cells snail1 immunostaining was detected in 75 of 110 cases (68%). Expression of the protein was more common in hypopharyngeal tumours (p= 0.044). Snail1 positive tumours associated with a lower Karnofsky performance status score (p= 0.039) and regional failure (p= 0.042). Our findings indicate that snail1 protein expression in endothelial cells and to some extent also in tumour stromal myofibroblasts seems to be a predictor of poor survival in PSCC. The presence of snail1 protein in tumour microenvironment rather than in malignant epithelial tumour cells may induce tissue remodelling and tumour progression.