Effects of carbon dots surface functionalities on cellular behaviors - Mechanistic exploration for opportunities in manipulating uptake and translocation
Effects of carbon dots surface functionalities on cellular behaviors - Mechanistic exploration for opportunities in manipulating uptake and translocation
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碳点表面功能对细胞行为的影响 - 操纵吸收和易位机会的机制探索
DOI：
10.1016/j.colsurfb.2019.05.027
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Wang Haifang
中科院分区：
文献类型：
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作者：
Yan Gui-Hua;Song Zheng-Mei;Liu Yuan-Yuan;Su Qianqian;Liang Weixiong;Cao Aoneng;Sun Ya-Ping;Wang Haifang
碳量子点（CDots）因其优异的光学和其他性质而在生物医学领域得到了广泛的应用。为此，选择了表面经3-乙氧基丙胺（EPA-CDots）和低聚聚乙烯亚胺（PEI-CDots）钝化的两种CDots，用于评价它们在三个细胞周期阶段（G 0/G1、S和G2/M）被人宫颈癌HeLa细胞摄取，以及它们在细胞中的不同内化途径和易位。结果表明，HeLa细胞可以通过不同的途径内化两种CDot，PEI-CDot的整体内化效率略高。培养基中血清的存在可具有主要作用，显著增强EPA-CDot的细胞摄取，但显著抑制PEI-CDot的细胞摄取。不同细胞周期时相的HeLa细胞对CDots的摄取行为不同，这也受到不同的点表面基团和培养基中血清的影响。机制的影响的结果和机会与CDots的细胞行为的潜在的操纵和控制其细胞的摄取和易位的改进的理解进行了讨论。
Carbon dots (CDots) for their excellent optical and other properties have been widely pursued for potential biomedical applications, in which a more comprehensive understanding on the cellular behaviors and mechanisms of CDots is required. For such a purpose, two kinds of CDots with surface passivation by 3-ethoxypropylamine (EPA-CDots) and oligomeric polyethylenimine (PEI-CDots) were selected for evaluations on their uptakes by human cervical carcinoma HeLa cells at three cell cycle phases (G0/G1, S and G2/M), and on their different internalization pathways and translocations in cells. The results show that HeLa cells could internalize both CDots by different pathways, with an overall slightly higher internalization efficiency for PEI-CDots. The presence of serum in culture media could have major effects, significantly enhancing the cellular uptake of EPA-CDots, yet markedly inhibiting that of PEI-CDots. The HeLa cells at different cell cycle phases have different behaviors in taking up the CDots, which are also affected by the different dot surface moieties and serum in culture media. Mechanistic implications of the results and the opportunities associated with an improved understanding on the cellular behaviors of CDots for potentially the manipulation and control of their cellular uptakes and translocations are discussed.