Global proteomic analysis in trypanosomes reveals unique proteins and conserved cellular processes impacted by arginine methylation.
Global proteomic analysis in trypanosomes reveals unique proteins and conserved cellular processes impacted by arginine methylation.
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DOI：
10.1016/j.jprot.2013.07.010
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发表时间：
2013-10-08
影响因子：
3.3
通讯作者：
Read LK
中科院分区：
文献类型：
--
作者：
Lott K;Li J;Fisk JC;Wang H;Aletta JM;Qu J;Read LK
精氨酸甲基化是一种常见的翻译后修饰，在转录、RNA加工和翻译以及DNA修复中具有重要作用。锥虫编码五种蛋白精氨酸甲基转移酶，表明精氨酸甲基化对这些生物体的生物学有广泛的影响。在这里，我们对布鲁氏杆菌进行了全球蛋白质组学分析，以确定精氨酸甲基化蛋白及其修饰位点。利用二维色谱分离、交替电子转移解离和碰撞诱导解离的方法，我们在676种蛋白质中鉴定出1332种甲基精氨酸。由此产生的数据集代表了迄今为止任何生物体中精氨酸甲基化蛋白的最大汇编。功能分类显示许多精氨酸甲基化蛋白参与鞭毛功能、RNA代谢、DNA复制和修复以及细胞内蛋白质运输。因此，精氨酸甲基化有可能影响布鲁氏杆菌基因表达、细胞生物学和发病机制。有趣的是，本研究中发现了高等真核生物中已知甲基化蛋白的途径，但通常该途径的不同成分在锥虫中被甲基化。甲基精氨酸通常在富含甘氨酸的环境中被识别出来，尽管也发现了例外情况。总的来说，这些数据为锥虫生物学的许多方面提供了信息，并为高等真核生物中同源精氨酸甲基化蛋白的鉴定提供了指导。
Arginine methylation is a common posttranslational modification with reported functions in transcription, RNA processing and translation, and DNA repair. Trypanosomes encode five protein arginine methyltransferases, suggesting that arginine methylation exerts widespread impacts on the biology of these organisms. Here, we performed a global proteomic analysis of T. brucei to identify arginine methylated proteins and their sites of modification. Using an approach entailing two-dimensional chromatographic separation, and alternating electron transfer dissociation and collision induced dissociation, we identified 1332 methylarginines in 676 proteins. The resulting data set represents the largest compilation of arginine methylated proteins in any organism to date. Functional classification revealed numerous arginine methylated proteins involved in flagellar function, RNA metabolism, DNA replication and repair, and intracellular protein trafficking. Thus, arginine methylation has the potential to impact aspects of T. brucei gene expression, cell biology, and pathogenesis. Interestingly, pathways with known methylated proteins in higher eukaryotes were identified in this study, but often different components of the pathway were methylated in trypanosomes. Methylarginines were often identified in glycine rich contexts, although exceptions to this rule were detected. Collectively, these data inform on a multitude of aspects of trypanosome biology and serve as a guide for the identification of homologous arginine methylated proteins in higher eukaryotes.
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影响因子：
3.7
作者：
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