The Rpb4/7 Module of RNA Polymerase II Is Required for Carbon Catabolite Repressor Protein 4-Negative on TATA (Ccr4-Not) Complex to Promote Elongation
The Rpb4/7 Module of RNA Polymerase II Is Required for Carbon Catabolite Repressor Protein 4-Negative on TATA (Ccr4-Not) Complex to Promote Elongation
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DOI：
10.1074/jbc.c114.601088
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发表时间：
2014-11-28
影响因子：
4.8
通讯作者：
Reese, Joseph C.
中科院分区：
文献类型：
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作者：
Babbarwal, Vinod;Fu, Jianhua;Reese, Joseph C.
基因表达依赖于细胞核中mRNA合成和细胞质中mRNA衰变之间的平衡，这两个过程曾经被认为是分开的。我们现在知道，转录和衰变率是协调的，但因素或分子机制尚不清楚。Ccr 4-Not复合物调节基因表达的多个阶段，从mRNA合成到蛋白质破坏。其功能之一是通过重新激活停滞的延伸复合物来促进RNA聚合酶II延伸。在这里，我们探索了Ccr 4-Not促进延伸所需的聚合酶的特征，并发现Rpb 4/7模块对于Ccr 4-Not与延伸复合物结合并刺激延伸是重要的。Rpb 4/7还参与协调mRNA的合成和衰变，但其在这一过程中的作用存在争议。Ccr 4-Not和Rpb 4/7之间的相互作用表明了细胞如何协调mRNA合成和衰变的机制。
Gene expression relies on the balance between mRNA synthesis in the nucleus and decay in the cytoplasm, processes once thought to be separate. We now know that transcription and decay rates are coordinated, but the factors or molecular mechanisms are unclear. The Ccr4-Not complex regulates multiple stages of gene expression, from mRNA synthesis to protein destruction. One of its functions is to promote RNA polymerase II elongation by reactivating arrested elongation complexes. Here we explored the features of polymerase required for Ccr4-Not to promote elongation and found that the Rpb4/7 module is important for Ccr4-Not to associate with elongation complexes and stimulate elongation. Rpb4/7 has also been implicated in coordinating mRNA synthesis and decay, but its role in this process is controversial. The interplay between Ccr4-Not and Rpb4/7 described here suggests a mechanism for how the cell coordinates mRNA synthesis and decay.