Expression of caveolin-1 is correlated with Akt-1 in colorectal cancer tissues
Expression of caveolin-1 is correlated with Akt-1 in colorectal cancer tissues
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DOI：
10.1016/j.yexmp.2005.09.001
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发表时间：
2006-04-01
影响因子：
3.6
通讯作者：
Lee, RA
中科院分区：
文献类型：
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作者：
Kim, HA;Kim, KH;Lee, RA
小窝蛋白是质膜微区小窝的主要成分。小窝蛋白在肿瘤发生中的新作用主要基于癌细胞系的体外实验。我们对caveolin、Akt和EGFR进行了半定量RT-PCR，以了解caveolin在结直肠肿瘤生物学中的作用。癌组织样本和邻近的正常结肠粘膜取自在梨花女子大学木洞医院接受手术的95名结直肠癌患者。用这些新鲜组织，通过用β-肌动蛋白共扩增小窝蛋白-1、EGFR和Akt-1的基因进行半定量RT-PCR。平均年龄为60.21 ± 13.33岁，性别比为1.44：1。Caveolin-1在肿瘤中的表达高于正常粘膜（P = 0.025）。Caveolin-1和Akt-1的表达存在明确的正相关（P = 0.002）。caveolin-1与EGFR的表达无明显相关性。未发现caveolin-1表达与临床因素相关。结论：caveolin-1在结肠癌组织中的表达高于正常结肠组织，且与Akt-1相关，而与EGFR表达无关，提示caveolin-1的信号转导影响Akt-1的活化，但该反应在结肠癌中不是由EGFR刺激启动的。(c)2005年爱思唯尔公司All rights reserved.
Caveolin is a major component of caveolae which is a plasma membrane microdomain. The emerging role of caveolin in tumorigenesis was based mainly on in vitro experiments with cancer cell lines. We performed semi-quantitative RT-PCR for caveolin, Akt and EGFR to understand the role of caveolins in colorectal tumor biology. Cancer tissue samples and the neighboring normal colon mucosa were obtained from 95 colorectal cancer patients who underwent operations at Ewha Womans University Mokdong Hospital. With these fresh tissues, semi-quantitative RT-PCR was performed by coamplification of the gene for caveolin-1, EGFR and Akt-1 with beta-actin. The average age was 60.21 +/- 13.33 years old, and sex ratio was 1.44:1. Caveolin-1 is more expressed in tumors than normal mucosa (P = 0.025). The expression of caveolin-1 and Akt-1 had a definitive positive relationship (P = 0.002). But, the expression of caveolin-1 and EGFR was not significantly related. We could not find correlations between caveolin-1 expression and clinical factors. In conclusion, caveolin-1 is more expressed in cancer tissues than normal colon and related with Akt-1, not with EGFR expression in colorectal cancer tissues, which suggests that signaling for caveolin-1 affects Akt-1 activation, but this reaction is not initiated by EGFR stimulation in colon cancer. (c) 2005 Elsevier Inc. All rights reserved.