EXPRESSION OF AEQUOREA GREEN FLUORESCENT PROTEIN IN PLANT-CELLS
EXPRESSION OF AEQUOREA GREEN FLUORESCENT PROTEIN IN PLANT-CELLS
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DOI：
10.1016/0014-5793(95)00776-6
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发表时间：
1995-08-07
期刊：
影响因子：
3.5
通讯作者：
CHENG, CL
中科院分区：
文献类型：
--
作者：
HU, W;CHENG, CL
维多利亚水母的绿色荧光蛋白（GFP）编码区已与花椰菜花叶病毒35 S启动子融合，并导入玉米叶原生质体中。观察到GFP的瞬时表达。此外，将GFP的编码区融合到拟南芥热休克启动子上，并与另一种构建体共转化，其中GFP已被氯霉素乙酰转移酶（CAT）取代。热诱导的GFP在玉米原生质体中的表达与CAT的表达平行。虽然GFP在黑暗生长和绿色玉米叶原生质体中均表达，但在类似转化的拟南芥原生质体中未观察到绿色荧光。
The coding region of the green fluorescent protein (GFP) from Aequorea victoria has been fused to the cauliflower mosaic virus 35S promoter and introduced into maize leaf protoplasts. Transient expression of GFP was observed. In addition, the coding region of GFP was fused to an Arabidopsis heat shock promoter and co-transformed with another construct in which GFP has been replaced with chloramphenicol acetyltransferase (CAT). The heat-induced expression of GFP in maize protoplasts parallels that of CAT. While GFP was expressed in both dark-grown and green maize leaf protoplasts, no green fluorescence was observed in similarly transformed Arabidopsis protoplasts.