In vivo bioprinting for computer- and robotic-assisted medical intervention: preliminary study in mice
In vivo bioprinting for computer- and robotic-assisted medical intervention: preliminary study in mice
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DOI：
10.1088/1758-5082/2/1/014101
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发表时间：
2010-03-01
期刊：
影响因子：
9
通讯作者：
Catros, Sylvain
中科院分区：
文献类型：
--
作者：
Keriquel, Virginie;Guillemot, Fabien;Catros, Sylvain
我们首次尝试将生物打印技术应用于计算机辅助医疗干预。设计了高通量生物激光打印工作站。在小鼠颅骨缺损模型中制备了纳米羟基磷灰石（n-HA）。用直径为4mm的环钻在OF-1雄性小鼠颅骨上制造临界大小的骨缺损。在激光打印实验之前，通过磁共振成像显示激光照射小鼠硬脑膜后无炎症反应。讨论了体内生物打印的程序和使用脱钙切片和x射线显微断层扫描获得的结果。尽管存在差异，但这些初步结果表明，体内生物打印是可能的。生物打印在未来可能会被证明对医疗机器人和计算机辅助医疗干预很有帮助。
We present the first attempt to apply bioprinting technologies in the perspective of computer-assisted medical interventions. A workstation dedicated to high-throughput biological laser printing has been designed. Nano-hydroxyapatite (n-HA) was printed in the mouse calvaria defect model in vivo. Critical size bone defects were performed in OF-1 male mice calvaria with a 4 mm diameter trephine. Prior to laser printing experiments, the absence of inflammation due to laser irradiation onto mice dura mater was shown by means of magnetic resonance imaging. Procedures for in vivo bioprinting and results obtained using decalcified sections and x-ray microtomography are discussed. Although heterogeneous, these preliminary results demonstrate that in vivo bioprinting is possible. Bioprinting may prove to be helpful in the future for medical robotics and computer-assisted medical interventions.