Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.
Cryopreservation of transgenic Huntington's disease rhesus macaque sperm-A Case Report.
复制标题
DOI：
10.4172/2168-9849.1000116
复制
发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Kittiphong Putkhao;A. Chan;Y. Agca;R. Parnpai
中科院分区：
文献类型：
--
作者：
Kittiphong Putkhao;A. Chan;Y. Agca;R. Parnpai
本文研究了甘油在三种不同的精液冷冻稀释剂Tris-柠檬酸盐(Tris)、TestTM和TES-Tris-蛋黄(TTE)中对野生(WT)恒河猴精子冷冻的保护作用。通过检测精子活力和存活率来评价冷冻解冻后精子的完整性，筛选出冷冻保存转基因亨廷顿病(HD)恒河猴精子的最佳稀释液。结果表明，不同冷冻延伸剂在解冻后的运动性能无显著差异(P>；0.05)。但T组和TTE组精子膜完整性明显好于Tris稀释液(P<0.05)。本研究表明，TEST和TTE是一种优良的精子冷冻稀释剂，适合于恒河猴精子的冷冻保存，试验稀释液对HD和WT恒河猴精子冷冻后精子活力和存活率无明显影响。
The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic Huntington's disease (HD) rhesus monkey. The results showed no post-thaw motility difference among the freezing extender tested (P>0.05). However, sperm membrane integrity in TEST and TTE were significantly better than in TRIS extender (P0.05). The present study demonstrates that TEST and TTE were excellent extenders and suitable for rhesus monkey sperm cryopreservation and no detectible differences of post-thaw sperm motility and viability between HD and WT rhesus monkeys resulted from TEST extender.