Persistence of Eimeria bovis in soil
Persistence of Eimeria bovis in soil
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DOI：
10.1007/s00436-013-3413-4
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发表时间：
2013-07-01
影响因子：
2
通讯作者：
Bangoura, Berit
中科院分区：
文献类型：
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作者：
Lassen, Brian;Lepik, Triin;Bangoura, Berit
艾美耳球虫病是一种全球性的疾病，经常影响在分别被致病性物种牛艾美耳球虫、祖尔尼艾美耳球虫或阿拉巴马艾美耳球虫的卵囊污染的牧场上放牧的牛。尽管如此，很少有人了解有关卵囊持久性的牧场。该研究在温带气候区进行。土壤样品中掺入100，000 E。2010年7月用牛卵囊或2010年10月用50，000个卵囊，两者都添加或不添加牛粪便。土壤样本暴露于自然环境条件下，直至2011年4月。加标后立即分析样品子集作为阳性对照。通过漂浮法回收卵囊，并在阅读室中计数。平均可从阳性对照中回收23%的卵囊。在7月制备的样品中，卵囊的回收率下降至原始水平的0.30%，与粪便的添加无关，而在10月制备的样品中，卵囊计数较高，不含（2.05%）和含（2.64%）粪便材料。在存在或不存在粪便的情况下，未观察到存在卵囊或回收的卵囊计数之间存在差异。粪便的存在对卵囊的完整性有积极的影响。在冬季，卵囊在土壤中的数量在大多数样品的检测限以下降低。另一方面，相对较短的3个月的夏季也对持续存在的卵囊数量有显着影响。
Eimeriosis is a disease that occurs globally and often affects cattle grazing on pastures contaminated with oocysts of the pathogenic species Eimeria bovis, Eimeria zuernii or Eimeria alabamensis, respectively. Nonetheless, little is understood regarding oocyst persistence on the pasture. The study was performed in the temperate climate zone. Soil samples were spiked with 100,000 E. bovis oocysts in July 2010 or with 50,000 oocysts in October 2010, respectively, both either with our without addition of cattle faeces. The soil samples were exposed to natural environmental conditions until April 2011. A subset of the samples was analysed immediately after spiking as positive control. The oocysts were recovered by a flotation method and counted in a reading chamber. On average, 23 % of the oocysts could be recovered from the positive control. The recovery of oocysts dropped to 0.30 % of the original level in the samples prepared in July independent of the addition of faeces, whereas the oocyst count was higher in the samples prepared in October, both without (2.05 %) and with (2.64 %) faecal material. No differences were observed between presence of oocysts or oocyst counts recovered in the presence or absence of faeces. Presence of faeces had a positive influence on oocyst integrity. During the winter season, the number of oocysts in the soil was lowered under the detection limit in most samples. On the other hand, the comparatively short 3-month summer period had a significant influence on the number of persisting oocysts too.