Implication of a Novel Function of Sar1 in the Nucleus
Implication of a Novel Function of Sar1 in the Nucleus
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DOI：
10.1247/csf.19019
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发表时间：
2019-01-01
影响因子：
1.5
通讯作者：
Sato, Ken
中科院分区：
文献类型：
--
作者：
Horikawa, Kotaro;Yorimitsu, Tomohiro;Sato, Ken
外壳蛋白复合物II（COPII）产生将新合成的蛋白质从内质网（ER）递送到高尔基体的转运载体。小GTSAR 1是COPII外套组件的知名调节器。在本研究中，我们证明，除了其公认的作用，ER-高尔基体贩运，核定位的Sar 1是必不可少的酿酒酵母的生存能力。抑制核进入或保留Sar 1导致严重的生长缺陷。此外，通过使用条件性遗传耗竭，在体内缺失Sar 1，进一步证明了Sar 1的核定位的损失导致异常的核膜形状。我们的研究结果强调了一个可能的新的作用，核内的Sar 1，这可能涉及到正确的核包膜的形成。
The coat protein complex II (COPII) generates transport carriers that deliver newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. The small GTPase Sar1 is a well-known regulator of the assembly of the COPII coat. In the present study, we demonstrate that, besides its well-established role in ER-to-Golgi trafficking, the nuclear localization of Sar1 is essential for the viability of Saccharomyces cerevisiae. Inhibition of either the nuclear entry or retention of Sar1 leads to a severe growth defect. Additionally, in vivo deletion of Sar1, by using conditional genetic depletion, further demonstrates that the loss of nuclear localization of Sar1 results in an abnormal nuclear envelope shape. Our findings highlighted a possible novel role of Sar1 within the nucleus, which may relate to the proper formation of the nuclear envelope.