Torovirus detection in faecal specimens of calves and pigs in hungary:: Short communication
Torovirus detection in faecal specimens of calves and pigs in hungary:: Short communication
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DOI：
10.1556/avet.50.2002.3.5
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发表时间：
2002-01-01
影响因子：
0.9
通讯作者：
Nagy, B
中科院分区：
文献类型：
--
作者：
Matiz, K;Kecskeméti, S;Nagy, B
牛圆环病毒是牛疾病的已知病原体，而猪圆环病毒只从健康动物中分离出来。环状病毒存在的证据已经在几个欧洲国家和美国得到了描述。在匈牙利进行了一项调查，通过对10头猪和9个牛群进行抽样来检测轮状病毒。采集腹泻犊牛和断奶仔猪的直肠拭子和粪便标本。用环状病毒特异性引物进行逆转录聚合酶链式反应(RT-PCR)检测，阳性标本进一步进行电子显微镜(EM)检查。在4头腹泻犊牛(111头)和10头健康断奶仔猪(200头健康断奶猪)中检测到环状病毒，代表9头犊牛群中的2头和10头猪群中的2头。这是匈牙利首例环状病毒确诊病例。
Bovine torovirus is an established aetiological agent of disease in cattle, while porcine torovirus has only been isolated from healthy animals. Evidence for the presence of torovirus has been described in several European countries and also in the United States. A survey was performed to detect toroviruses in Hungary by means of sampling ten swine and nine bovine herds. Rectal swabs and faecal specimens were collected from diarrhoeic calves and from weaned piglets. The samples were tested by the reverse transcription-polymerase chain reaction (RT-PCR) using torovirus-specific primers and the positive samples were further examined by electron microscopy (EM). Torovirus was detected in 4 diarrhoeic calves (out of 111) and in 10 healthy weaned pigs (out of 200 tested), representing two of the 9 calf herds and two of the 10 pig herds tested. This is the first report of exact diagnosis of torovirus in Hungary.