ULTRASTRUCTURAL FEATURES OF FETAL ERYTHROID PRECURSORS INFECTED WITH PARVOVIRUS-B19 INVITRO - EVIDENCE OF CELL-DEATH BY APOPTOSIS
ULTRASTRUCTURAL FEATURES OF FETAL ERYTHROID PRECURSORS INFECTED WITH PARVOVIRUS-B19 INVITRO - EVIDENCE OF CELL-DEATH BY APOPTOSIS
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DOI：
10.1002/path.1711690207
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发表时间：
1993-02-01
影响因子：
7.3
通讯作者：
FLEMING, KA
中科院分区：
文献类型：
--
作者：
MOREY, AL;FERGUSON, DJP;FLEMING, KA
人类细小病毒B19不能在标准细胞系中培养，对该病毒的细胞内生命周期知之甚少。本研究用人胎肝造血细胞悬液培养法观察了B19感染的超微结构特征。来自受感染的培养物的红细胞经常含有充满和空的病毒样颗粒的结晶阵列。随着培养时间的延长，这些排列的数量和大小逐渐增加，其位置在感染后24小时由单纯的核变为感染后3天的核和胞质并存。有时在细胞质突起中发现排列，这些突起似乎是从细胞中掐掉的。阵列的位置与免疫标记在显微镜水平上确定的病毒衣壳蛋白的分布相对应。含有病毒晶体阵列的细胞也表现出核仁变性、核异染色质极端边际化和细胞质空泡化。这些特征是经历个别程序性细胞死亡或“凋亡”的细胞的典型特征。细小病毒B19引起的红系前体细胞的凋亡可能有助于解释胎儿B19感染缺乏强烈的炎症反应，并可能对了解病毒在宿主中传播的机制有一定的意义。
Human parvovirus B19 cannot be cultured in standard cell lines and relatively little is known about the intracellular life-cycle of the virus. In this study, ultrastructural features of B19 infection were examined using haemopoietic cell suspension cultures derived from human fetal liver. Erythroblasts from infected cultures frequently contained crystalline arrays of both full and empty virus-like particles. The number and size of these arrays increased with the duration of culture, and their location changed from exclusively nuclear at 24 h post-infection to both nuclear and cytoplasmic at 3 days post-infection. Arrays were occasionally found in cytoplasmic protuberances which appeared to be pinching off from the cell. The location of the arrays corresponded to the distribution of viral capsid protein determined by immunolabelling at the fight microscope level. Cells containing viral crystalline arrays also exhibited nucleolar degeneration, extreme margination of the nuclear heterochromatin, and cytoplasmic vacuolation. These features are typical of cells undergoing individual programmed cell death or 'apoptosis'. The triggering of apoptosis in erythroid precursors by parvovirus B19 may help to explain the apparent lack of a strong inflammatory response to fetal B19 infection and may have implications for understanding the mechanisms of viral spread throughout the host.