The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay
The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay
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DOI：
10.1006/abio.1996.0292
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发表时间：
1996-07-15
影响因子：
2.9
通讯作者：
Strain, JJ
中科院分区：
文献类型：
--
作者：
Benzie, IFF;Strain, JJ
一种简单的，自动化的测试测量血浆铁还原能力，FRAP测定，提出了作为一种新的方法来评估“抗氧化能力。在低pH值下，亚铁离子还原为亚铁离子，会形成有色的亚铁-三吡啶基三嗪络合物。FRAP值是通过比较试验反应混合物与含有已知浓度亚铁离子的混合物在593 nm处的吸光度变化而获得的。吸光度变化在宽浓度范围内与抗氧化剂混合物（包括血浆）和含有一种纯化形式抗氧化剂的溶液呈线性关系。抗氧化剂之间没有明显的相互作用。测得的Trolox、α-生育酚、抗坏血酸和尿酸的化学计量因子均为2.0;胆红素的化学计量因子为4.0。白蛋白的活性很低。运行内和运行间CV为
A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing ''antioxidant power.'' Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, alpha-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are