Non-invasive live-cell measurement of chancres in macrophage NAD(P)H by two-photon microscopy
Non-invasive live-cell measurement of chancres in macrophage NAD(P)H by two-photon microscopy
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DOI：
10.1016/j.imlet.2003.12.013
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发表时间：
2005-01-15
影响因子：
4.4
通讯作者：
Kiemer, AK
中科院分区：
文献类型：
--
作者：
Kable, EPW;Kiemer, AK
双光子显微镜允许使用长波长光确定UV可激发的荧光团。我们的目的是确定NAD（P）H（P）H自发荧光作为巨噬细胞NADPH氧化酶激活的量度。RAW264.7巨噬细胞在盖玻片上生长并保存在HBSS中用于显微镜研究。用710nm光激发细胞并检测NAD（P）H自发荧光。葡萄糖和NaCN诱发NAD（P）H自发荧光增加。NADPH氧化酶的激活剂导致NAD（P）H自发荧光显著降低。因此，这项工作表明，双光子显微镜作为一种非侵入性的方法，确定激活后吞噬细胞NAD（P）H的变化的适用性。(C)2004 Elsevier B.V.保留所有权利。
Two-photon microscopy allows determination of UV-excitable fluorophores using long-wavelength light. We aimed to determine NAD(P)H(P)H autofluorescence as a measure for macrophage NADPH-oxidase activation. RAW264.7 macrophages were grown on glass coverslips and kept in HBSS for microscopic investigation. Cells were excited with 710nm light and NAD(P)H autofluorescence was detected. Glucose as well as NaCN evoked an increase of NAD(P)H autofluorescence. Activators of NADPH oxidase lead to significantly decreased NAD(P)H autofluorescence. Therefore, this work shows the suitability of two-photon microscopy as a non-invasive method determining changes in phagocyte NAD(P)H upon activation. (C) 2004 Elsevier B.V. All rights reserved.