Potential of Hydrogels Based on Poly(Ethylene Glycol) and Sebacic Acid as Orthopedic Tissue Engineering Scaffolds
Potential of Hydrogels Based on Poly(Ethylene Glycol) and Sebacic Acid as Orthopedic Tissue Engineering Scaffolds
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DOI：
10.1089/ten.tea.2008.0326
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发表时间：
2009-08-01
影响因子：
4.1
通讯作者：
Lu, Lichun
中科院分区：
文献类型：
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作者：
Kim, Jinku;Hefferan, Theresa E.;Lu, Lichun
本研究检测了聚乙二醇戊二酸二丙烯酸酯(PEGSDA)水凝胶修饰前后对骨髓基质细胞(MSCs)成骨分化和矿化的影响。在另一项单独的实验中，还研究了PEGSDA水凝胶作为骨形态发生蛋白2(BMP-2)载体的能力。作为支架材料，经RGD多肽修饰前后间充质干细胞在PEGSDA水凝胶支架上的附着和增殖情况与对照、组织培养聚苯乙烯相似。相比之下，在长达21天的培养期间，未经修饰的聚乙二醇二丙烯酸酯(PEGDA)水凝胶上几乎看不到细胞。PEGSDA水凝胶的成骨表型表达，如碱性磷酸酶(ALP)和矿化钙含量明显高于对照组和PEGDA水凝胶。PEGSDA支架作为骨形成分子如BMP-2的输送载体的潜在用途也被评估。在21天释放期的前3天，PEGSDA水凝胶支架的BMP-2初始释放量(14.7%)明显低于PEGDA水凝胶支架(84.2%)。PEGSDA水凝胶支架中释放的BMP-2与PEGDA支架中释放的BMP-2相比，成骨细胞的碱性磷酸酶活性显著升高，尤其是在释放6天后。总体而言，未经进一步修饰的PEGSDA水凝胶支架可用作骨科组织工程支架和局部药物载体，以延长骨诱导分子的持续释放。
In this study, the bioactive effects of poly( ethylene glycol) ( PEG) sebacic acid diacrylate (PEGSDA) hydrogels with or without RGD peptide modification on osteogenic differentiation and mineralization of marrow stromal cells (MSCs) were examined. In a separate experiment, the ability of PEGSDA hydrogel to serve as a delivery vehicle for bone morphogenetic protein 2 (BMP-2) was also investigated. As a scaffold, the attachment and proliferation of MSCs on PEGSDA hydrogel scaffolds with and without RGD peptide modification was similar to the control, tissue culture polystyrene. In contrast, cells were barely seen on unmodified PEG diacrylate (PEGDA) hydrogel throughout the culture period for up to 21 days. Osteogenic phenotypic expression such as alkaline phosphatase ( ALP) of MSCs as well as mineralized calcium content were significantly higher on PEGSDA-based hydrogels than those on the control or PEGDA hydrogels. Potential use of PEGSDA scaffold as a delivery vehicle of osteogenic molecules such as BMP-2 was also evaluated. Initial burst release of BMP-2 from PEGSDA hydrogel scaffold (14.7%) was significantly reduced compared to PEGDA hydrogel scaffold (84.2%) during the first 3 days of a 21-day release period. ALP activity of an osteoblast was significantly higher in the presence of BMP-2 released from PEGSDA hydrogel scaffolds compared to that in the presence of BMP-2 released from PEGDA scaffolds, especially after 6 days of release. Overall, PEGSDA hydrogel scaffolds without further modification may be useful as orthopedic tissue engineering scaffolds as well as local drug carriers for prolonged sustained release of osteoinductive molecules.