Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System
Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System
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DOI：
10.3390/s100100697
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发表时间：
2010-01-01
期刊：
影响因子：
3.9
通讯作者：
Lee, Da-Sheng
中科院分区：
文献类型：
--
作者：
Lee, Da-Sheng
基于芯片的DNA定量系统被广泛使用，并在许多医疗保健应用中使用。但是，用于此类应用的仪器可能不会定期维护或校准。由于机器的可靠性是机器正常运行的关键问题，本研究提出了实时聚合酶链式反应(PCR)机器的系统模型，通过数值实验来分析仪器的设计。在模型分析的基础上，开发了一种系统的方法来降低DNA定量的变化，并实现实时芯片上聚合酶链式反应系统的稳健设计。采用加速升降试验对芯片样机的可靠性进行评估。根据寿命测试计划，该实时芯片聚合酶链式反应系统被模拟连续工作三年以上，在DNA定量方面具有类似的重复性。这不仅显示了芯片实验室系统的健壮性，也验证了我们实现健壮性设计的系统方法的有效性。
Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.