Accelerated atrazine degradation and altered metabolic pathways in goat manure assisted soil bioremediation
Accelerated atrazine degradation and altered metabolic pathways in goat manure assisted soil bioremediation
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山羊粪便辅助土壤生物修复中莠去津的加速降解和代谢途径的改变
DOI：
10.1016/j.ecoenv.2021.112432
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发表时间：
2021
影响因子：
6.8
通讯作者：
Liang Yan-Qiu
中科院分区：
文献类型：
--
作者：
Luo Shuwen;Zhen Zhen;Zhu Xiaoping;Ren Lei;Wu Weijian;Zhang Weijian;Chen Yijie;Zhang Dayi;Song Zhiguang;Lin Zhong;Liang Yan-Qiu
阿特拉津在农业生产中的大量、长期使用已造成严重的环境污染，危害生态系统和人类健康。土壤微生物在阿特拉津降解过程中起着重要作用。然而，由于其生长缓慢、丰度低，其降解效率相对较低，通过施用有机肥来改善土壤养分和微生物活性可以解决这些问题。研究了羊粪对阿特拉津降解性能、代谢产物和细菌群落结构的影响。结果表明，阿特拉津在未施用有机肥的土壤中的降解率为26.9%-35.7%，施用羊粪后阿特拉津的降解率提高到60.9%-84.3%。羟基阿特拉津途径没有显着改变，而脱乙基阿特拉津和脱异丙基阿特拉津途径显着增强与粪肥修订的处理，通过鼓励阿特拉津侧链的N-脱烷基化。羊粪显著提高了土壤pH值、有机质和腐殖质含量，说明阿特拉津代谢途径发生了变化;类诺卡氏菌、鞘氨醇单胞菌和球孢菌与阿特拉津降解效率和3种代谢产物呈正相关，表明它们在阿特拉津污染土壤中具有较好的选择性，可能在阿特拉津降解过程中发挥作用。研究结果表明，山羊粪既可作为菌剂又可作为营养物质改善土壤微环境，其改良可加速阿特拉津在污染场地的降解，为除草剂污染土壤的修复提供一种高效、廉价、生态友好的策略。
The intensive and long-term use of atrazine in agriculture has resulted in serious environmental pollution and consequently endangered ecosystem and human health. Soil microorganisms play an important role in atrazine degradation. However, their degradation efficiencies are relatively low due to their slow growth and low abundance, and manure amendment as a practice to improve soil nutrients and microbial activities can solve these problems. This study investigated the roles of goat manure in atrazine degradation performance, metabolites and bacterial community structure. Our results showed that atrazine degradation efficiencies in un-amended soils were 26.9–35.7% and increased to 60.9–84.3% in goat manure amended treatments. Hydroxyatrazine pathway was not significantly altered, whereas deethylatrazine and deisopropylatrazine pathways were remarkably enhanced in treatments amended with manure by encouraging the N-dealkylation of atrazine side chains. In addition, goat manure significantly increased soil pH and contents of organic matters and humus, explaining the change of atrazine metabolic pathway.Nocardioides,SphingomonasandMassiliawere positively correlated with atrazine degradation efficiency and three metabolites, suggesting their preference in atrazine contaminated soils and potential roles in atrazine degradation. Our findings suggested that goat manure acts as both bacterial inoculum and nutrients to improve soil microenvironment, and its amendment is a potential practice in accelerating atrazine degradation at contaminated sites, offering an efficient, cheap, and eco-friendly strategy for herbicide polluted soil remediation.