A set of BEST triple-resonance experiments for time-optimized protein resonance assignment
A set of BEST triple-resonance experiments for time-optimized protein resonance assignment
复制标题
DOI：
10.1016/j.jmr.2007.04.002
复制
发表时间：
2007-07-01
影响因子：
2.2
通讯作者：
Brutscher, Bernhard
中科院分区：
文献类型：
--
作者：
Lescop, Ewen;Schanda, Paul;Brutscher, Bernhard
提出了一系列连续的、残基内和双向的最佳H-N-CA、H-N-CO和H-N-CB脉冲序列，扩展了最近引入的快速多维蛋白质核磁共振的最佳概念[Schanda等人，J.Am化学。SoC。128(2006)9042]到顺序共振分配所需的全套实验。我们证明，对于蛋白质泛素，可以在配备低温探头的600 MHz核磁共振波谱仪上记录3D最佳H-N-C关联光谱，仅需几分钟的采集时间，并具有足够的灵敏度来检测所有预期的交叉峰。(C)2007 Elsevier Inc.保留所有权利。
A series of sequential, intra-residue, and bi-directional BEST H-N-CA, H-N-CO, and H-N-CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H-N-C correlation spectra can be recorded on a 600 MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks. (C) 2007 Elsevier Inc. All rights reserved.