Regulation of synaptotagmin gene expression during ascidian embryogenesis
Regulation of synaptotagmin gene expression during ascidian embryogenesis
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DOI：
10.1006/dbio.2002.0584
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发表时间：
2002-04-15
影响因子：
2.7
通讯作者：
Okamura, Y
中科院分区：
文献类型：
--
作者：
Katsuyama, Y;Matsumoto, J;Okamura, Y
海鞘胚胎是脊索动物发育的原始模式的模型，它迅速形成由少量神经元组成的背神经系统。在这里，我们的特点是海鞘突触结合蛋白（syt）基因的转录调控，探索突触传递的分子机制。原位杂交结果表明，syt表达在所有神经元在以前的研究和短暂的胚胎表皮。syt的神经元表达需要从胚胎的植物侧诱导，而表皮表达在分离的外胚层卵裂球中自主发生。将绿色荧光蛋白报告基因构建体导入海鞘胚胎中，结果表明，在其5'端上游3.4 kb的基因组片段中含有syt基因的启动子元件。启动子的缺失分析表明，syt在神经元和胚胎表皮中的表达依赖于不同的顺式调控区域。(C)2002 Elsevier Science（美国）。
The ascidian embryo, a model for the primitive mode of chordate development, rapidly forms a dorsal nervous system which consists of a small number of neurons. Here, we have characterized the transcriptional regulation of an ascidian synaptotagmin (syt) gene to explore the molecular mechanisms underlying development of synaptic transmission. In situ hybridization showed that syt is expressed in all neurons described in previous studies and transiently in the embryonic epidermis. Neuronal expression of syt requires induction from the vegetal side of the embryo, whereas epidermal expression occurs autonomously in isolated ectodermal blastomeres. Introduction of green fluorescent protein reporter gene constructs into the ascidian embryos indicates that a genomic fragment of the 3.4-kb 5' upstream region contains promoter elements of syt gene. Deletion analysis of the promoter suggests that syt expression in neurons and in the embryonic epidermis depends on distinct cis-regulatory regions. (C) 2002 Elsevier Science (USA).