Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny
Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny
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DOI：
10.1155/2013/784629
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发表时间：
2013-01-01
影响因子：
4.3
通讯作者：
Liebau, Stefan
中科院分区：
文献类型：
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作者：
Linta, Leonhard;Stockmann, Marianne;Liebau, Stefan
离子通道参与多种细胞过程，包括干细胞分化。生物体中存在许多离子通道家族，它们可以通过例如离子选择性、门控机制、组成或细胞生物学功能来区分。为了表征这组离子通道的不同表达，我们比较了人角质形成细胞来源的诱导多能干细胞(HiPSCs)及其体细胞来源的人头发角质形成细胞离子通道基因的mRNA表达水平。这一比较显示，26%的分析探针显示HiPSCs离子通道上调，而只有6%下调。此外，与角质形成细胞相比，IPSCs表达的离子通道数量要多得多。此外，为了缩小离子通道在iPS细胞中表达的特异性，我们比较了它们与分化的后代，即来自iPS细胞的神经元和心肌细胞的表达模式。综上所述，HiPSCs表现出非常丰富和多样化的离子通道表达模式。他们的详细分析可以让我们深入了解它们对许多细胞过程甚至疾病机制的贡献。
Ion channels are involved in a large variety of cellular processes including stem cell differentiation. Numerous families of ion channels are present in the organism which can be distinguished by means of, for example, ion selectivity, gating mechanism, composition, or cell biological function. To characterize the distinct expression of this group of ion channels we have compared the mRNA expression levels of ion channel genes between human keratinocyte-derived induced pluripotent stem cells (hiPSCs) and their somatic cell source, keratinocytes from plucked human hair. This comparison revealed that 26% of the analyzed probes showed an upregulation of ion channels in hiPSCs while just 6% were downregulated. Additionally, iPSCs express a much higher number of ion channels compared to keratinocytes. Further, to narrow down specificity of ion channel expression in iPS cells we compared their expression patterns with differentiated progeny, namely, neurons and cardiomyocytes derived from iPS cells. To conclude, hiPSCs exhibit a very considerable and diverse ion channel expression pattern. Their detailed analysis could give an insight into their contribution to many cellular processes and even disease mechanisms.