The control of class II expression on T cells is independent of the regulation of Tac and the induction of proliferation.
The control of class II expression on T cells is independent of the regulation of Tac and the induction of proliferation.
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T 细胞上 II 类表达的控制独立于 Tac 的调节和增殖的诱导。
DOI：
10.1111/j.1365-2249.1991.tb02967.x
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发表时间：
1991
影响因子：
4.6
通讯作者：
Zier,KS
中科院分区：
文献类型：
--
作者：
Salvadori,S;Pizzimenti,A;Cohen,S;Zier,KS
为了确定II类表达和T细胞活化的其他标志物之间的关联，我们测试了各种刺激模式诱导II类Tac表达和刺激增殖的能力。刺激的T细胞与植物血凝素（PHA）在辅助细胞的存在下强烈诱导增殖，Tac和II类抗原DR。当纯化的T细胞没有辅助细胞刺激佛波酯，PdB，和钙离子载体，离子霉素。诱导了强增殖和Tac表达，但仅观察到低水平的表面II类。相比之下，用PHA刺激相同的细胞导致弱增殖，强Tac，但再次低II类水平。加入PdB的PHA增加了增殖反应，但不影响Tac的表达，仍然很高，或II类表达，仍然很低。随后的培养在条件培养基中的纯化的T细胞已被激活与PdB和离子霉素或PHA导致增加的表面II类水平在这两种情况下。另外的实验表明，IL-2、IL-4和干扰素-γ（IFN-γ）都不是单独的原因。这些结果表明，II类表达可以从增殖的诱导和Tac的上调中分离出来，并且T细胞刺激的模式影响所得的活化途径。此外，他们认为，T细胞上II类表达的控制比其他细胞上的控制更严格。
In order to define the association between class II expression and other markers of T cell activation we tested the ability of various modes of stimulation to induce the expression of class II, Tac, and to stimulate proliferation. Stimulation of T cells with phytohaemagglutinin (PHA) in the presence of accessory cells strongly induced proliferation, Tac and the class II antigen DR. When purified T cells without accessory cells were stimulated with the phorbol ester, PdB, and the calcium ionophore, ionomycin. strong proliferation and Tac expression were induced, but only low levels of surface class II were observed. In contrast, stimulation of the same cells with PHA resulted in weak proliferation, strong Tac, but again low class II levels. The addition of PdB to the PHA increased the proliferative response, but did not affect Tac expression, which remained high, or class II expression, which remained low. Subsequent culture in conditioned medium of purified T cells which had been activated with either PdB and ionomycin or with PHA resulted in increased surface class II levels in both cases. Additional experiments suggested that neither IL-2, IL-4, nor interferon-gamma (IFN-γ) alone was responsible. These results demonstrate that class II expression can be separated from the induction of proliferation and the upregulation of Tac and that the mode of T cell stimulation influences the resulting activation pathway. Furthermore, they suggest that the control of class II expression on T cells is more tightly regulated than it is on other cells.