Adjuvant-induced arthritis:: IL-1β, IL-6 and TNF-α are up-regulated in the spinal cord
Adjuvant-induced arthritis:: IL-1β, IL-6 and TNF-α are up-regulated in the spinal cord
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DOI：
10.1097/00001756-200112210-00010
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发表时间：
2001-12-21
期刊：
影响因子：
1.7
通讯作者：
Lindgren, JU
中科院分区：
文献类型：
--
作者：
Bao, L;Zhu, Y;Lindgren, JU
佐剂性关节炎(AIA)是一种广泛使用的人类类风湿关节炎(RA)动物模型。我们先前已经证明在AIA大鼠的脊髓中观察到神经肽表达增加。为探讨细胞因子在AIA大鼠脊髓中的作用，本研究旨在探讨AIA大鼠脊髓中胶质细胞和细胞因子(IL-β、IL-6、TNF-α和干扰素-γ)表达的变化。结果表明，大鼠脑内巨胶质细胞和MHC-11免疫染色增强，表达GFAP的星形胶质细胞数量增多，染色强度增强。用原位杂交和免疫组织化学方法检测，关节炎大鼠脊髓组织中IL-Iβ、IL-6和TNF-α的mRNA和蛋白水平均显著升高。细胞因子在反应性星形胶质细胞和小胶质细胞中表达增加。《神经报告》12：3905-3908(C)2001 Lippincott Williams&Wilkins.
Adjuvant-induced arthritis (AIA) is a widely used animal model of human rheumatoid arthritis (RA). We have previously shown that increased neuropeptide expression is observed in the spinal cord of AIA rats. To study the potential role of cytokines in the spinal cord of AIA, we wanted to determine whether there are changes of glial and cytokine expression (IL- beta, IL-6, TNF-alpha and IFN-gamma) in the spinal cord of AIA rats. Our data indicated that macroglia and MHC class 11 immunostaining were enhanced, astrocytes expressing GFAP were increased in number and immunostaining intensity. Using in situ hybridization and immunohistochemical methods, both mRNA and protein levels of IL-I beta, IL-6 and TNF-alpha were significantly increased in the spinal cord of arthritic rats. Increased cytokine expression was presented in the reactive astrocytes and microglia. NeuroReport 12:3905-3908 (C) 2001 Lippincott Williams & Wilkins.