[Influence of storing conditions on the amino acid concentration in human serum (author's transl)].
[Influence of storing conditions on the amino acid concentration in human serum (author's transl)].
复制标题
[储存条件对人血清中氨基酸浓度的影响（作者翻译）]。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
M. Yamaguchi
中科院分区：
文献类型：
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作者：
K. Olek;S. Uhlhaas;P. Wardenbach;M. Yamaguchi
全血、血清和脱蛋白血清在室温下保存至24小时，在-30℃保存至1个月。用全自动氨基酸分析仪测定氨基酸含量。全血在室温放置时，瓜氨酸、α-氨基丁酸、半胱氨酸和色氨酸的浓度不变，而其他氨基酸则有显著的增加或减少。在室温下保存24 h的血清中，仅天冬氨酸、谷氨酸、丝氨酸、半胱氨酸和苯丙氨酸的浓度变化不明显。除天冬氨酸和谷氨酸外，脱蛋白血清可以在室温下放置24小时，在-30℃下放置1个月，而不会观察到其他氨基酸的浓度变化。当血清在-30摄氏度下储存24小时时，没有变化。
Whole blood, serum and deproteinized serum were stored at room temperature up to 24 h and at - 30 degrees C up to one month. The amino acid content was then determined with an automatic amino acid analyser. When whole blood is left at room temperature the concentration of citrulline, alpha-aminobutyric acid, cysteine and tryptophan remains unchanged, whereas the other amino acids show a remarkable increase or decrease. In serum stored for 24 h at room temperature, only the concentrations of aspartic and glutamic acid, serine, cysteine and phenylalanine were altered. With the exception of aspartic and glutamic acid it was possible to leave deproteinized serum up to 24 h at room temperature and up to one month at - 30 degrees C without observing a change in the concentration of the other amino acids. No change occurred, when serum was stored at - 30 degrees C for 24 h.