HOST-PATHOGEN INTERACTIONS .25. ENDOPOLYGALACTURONIC ACID LYASE FROM ERWINIA-CAROTOVORA ELICITS PHYTOALEXIN ACCUMULATION BY RELEASING PLANT-CELL WALL FRAGMENTS
HOST-PATHOGEN INTERACTIONS .25. ENDOPOLYGALACTURONIC ACID LYASE FROM ERWINIA-CAROTOVORA ELICITS PHYTOALEXIN ACCUMULATION BY RELEASING PLANT-CELL WALL FRAGMENTS
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DOI：
10.1104/pp.74.1.52
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发表时间：
1984-01-01
期刊：
影响因子：
7.4
通讯作者：
ALBERSHEIM, P
中科院分区：
文献类型：
--
作者：
DAVIS, KR;LYON, GD;ALBERSHEIM, P
Heat-labile elicitors of phytoalexin accumulation of soybeans (Glycine max L. Merr. cv. Wayne) were detected in culture filtrates of E. carotovora grown on a defined medium containing citrus pectin as the sole C source. The heat-labile elicitors were highly purified by cation-exchange chromatography on a CM-Sephadex (C-50) column, followed by agarose-affinity chromatography on a Bio-Gel A-0.5 m gel filtration column. The heat-labile elicitor activity co-purified with 2 .alpha.-1,4-endopolygalacturonic acid lyases (EC 4.2.2.2). Endopolygalacturonic acid lyase activity appeared to be necessary for elicitor activity because heat-inactivated enzyme preparations did not elicit phytoalexins. The purified endopolygalacturonic acid lyases elicited pterocarpan phytoalexins at microbial-inhibitory concentrations in the soybean-cotyledon bioassay when applied at a concentration of 55 ng/ml (1 .times. 10-9 M). One of these lyases released heat-stable elicitors from soybean cell walls, pectin and sodium polypectate. The heat-stable elicitor-active material solubilized from soybean cell walls by the lyase was composed of at least 90% (wt/vol) uronosyl residues. Endopolygalacturonic acid lyase elicits phytoalexin accumulation by releasing fragments from pectic polysaccharides in plant cell walls.
