Polarization of PI3K activity initiated by ooplasmic segregation guides nuclear migration in the mesendoderm.
Polarization of PI3K activity initiated by ooplasmic segregation guides nuclear migration in the mesendoderm.
复制标题
由卵质分离引发的 PI3K 活性极化引导中内胚层中的核迁移。
DOI：
10.1016/j.devcel.2015.10.012
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发表时间：
2015
影响因子：
11.8
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Takatori;N.;Oonuma;K.;Nishida;H. and Saiga;H.
RNA的不对称定位是广泛观察到的细胞极化机制。使用胚胎的海鞘，Halocynthia roretzi，我们以前表明，中胚层和内胚层的命运是分开的定位的mRNA编码的转录因子，不，未来的中胚层侧细胞质的中内胚层细胞通过不对称定位的细胞核。在这里，我们研究了定义核迁移方向的机制。我们发现PtdIns（3，4，5）P3定位于未来的中胚层区域决定了核迁移的方向。PtdIns（3，4，5）P3的定位依赖于PI3Kα的定位。PI 3K α首先定位于1细胞期的卵质运动。4细胞期的PI 3K α活性是PI 3K α定位至核迁移所必需的。我们的研究结果为理解导致胚层命运分离的因果关系链提供了框架。
Asymmetric localization of RNA is a widely observed mechanism of cell polarization. Using embryos of the ascidian,Halocynthia roretzi, we previously showed that mesoderm and endoderm fates are separated by localization of mRNA encoding a transcription factor,Not, to the future mesoderm-side cytoplasm of the mesendoderm cell through asymmetric positioning of the nucleus. Here, we investigated the mechanism that defines the direction of the nuclear migration. We show that localization of PtdIns(3,4,5)P3to the future mesoderm region determines the direction of nuclear migration. Localization of PtdIns(3,4,5)P3was dependent on the localization of PI3Kα to the future mesoderm region. PI3Kα was first localized at the 1-cell stage by the ooplasmic movement. Activity of localized PI3Kα at the 4-cell stage was required for the localization of PI3Kα up to the nuclear migration. Our results provide the scaffold for understanding the chain of causality leading to the separation of germ layer fates.