Integration of Brassica A genome genetic linkage map between Brassica napus and B. rapa

Integration of Brassica A genome genetic linkage map between Brassica napus and B. rapa
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DOI:
10.1139/g07-113
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发表时间:
2008-03-01
期刊:
影响因子:
3.1
通讯作者:
Bancroft, Ian
Bancroft, Ian
中科院分区:
生物学3区
文献类型:
--
作者:
Suwabe, Keita;Morgan, Colin;Bancroft, Ian

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基于总共 44 个共同标记,包括 41 个 SSR(33 个 BRMS、6 个 Saskatoon 和 2 个 BBSRC)和 3 个 SNP/indel 标记,构建了甘蓝型油菜和白菜油之间的整合连锁图。 3 到 7 个共同标记被映射到每个连锁群 A1 到 A10 上。最常见标记的位置和顺序揭示了物种之间的高度共线性,尽管 A4、A5 和 A10 上的两个小区域显示它们之间存在明显的局部反转。这些结果表明,尽管每个物种在A和C基因组以双二倍体状态整合后独立进化,但芸苔属的A基因组在物种之间保留了高度的共线性。我们的结果提供了甘蓝型油菜和白菜之间芸苔A基因组的遗传整合。由于分析采用了基于序列的分子标记,这些信息将加速利用白菜基因组序列来改良油菜。
An integrated linkage map between B. napus and B. rapa was constructed based on a total of 44 common markers comprising 41 SSR (33 BRMS, 6 Saskatoon, and 2 BBSRC) and 3 SNP/indel markers. Between 3 and 7 common markers were mapped onto each of the linkage groups A1 to A10. The position and order of most common markers revealed a high level of colinearity between species, although two small regions on A4, A5, and A10 revealed apparent local inversions between them. These results indicate that the A genome of Brassica has retained a high degree of colinearity between species, despite each species having evolved independently after the integration of the A and C genomes in the amphidiploid state. Our results provide a genetic integration of the Brassica A genome between B. napus and B. rapa. As the analysis employed sequence-based molecular markers, the information will accelerate the exploitation of the B. rapa genome sequence for the improvement of oilseed rape.