Arsenic Exposure Induces Genomic Hypermethylation
Arsenic Exposure Induces Genomic Hypermethylation
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DOI：
10.1002/tox.20497
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发表时间：
2010-06-01
影响因子：
4.5
通讯作者：
Dasgupta, Uma B.
中科院分区：
文献类型：
--
作者：
Majumdar, Sunipa;Chanda, Sarmishtha;Dasgupta, Uma B.
基因特异性高甲基化以前曾在砷暴露者中检测到。为监测不同砷暴露人群的全基因组甲基化水平，从64名受试者外周血单个核细胞中提取DNA。在DNA与Sssl甲基化酶温育后，测量从H-3标记的S-腺苷甲基化酶中摄取甲基。结果显示，在统计学上显着（P = 0.0004）的H-3甲基基团的摄取减少，在人接触到250-500 μ g/L砷，表明基因组甲基化。(C)2009 Wiley Periodicals，Inc.环境毒理学25：315-318，2010年。
Gene-specific hypermethylation has previously been detected in Arsenic exposed persons. To monitor the level of whole genome methylation in persons exposed to different levels of Arsenic via drinking water, DNA was extracted from peripheral blood mononuclear cells of 64 persons. Uptake of methyl group from H-3 labeled S-Adenosyl Methionine after incubation of DNA with Sssl methylase was measured. Results showed statistically significant (P = 0.0004) decrease in uptake of H-3 methyl group in the persons exposed to 250-500 mu g/L arsenic, indicating genomic hypermethylation. (C) 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 315-318, 2010.