The protective immune response of yellowtail Seriola quinqueradiata to the bacterial fish pathogen Lactococcus garvieae.
The protective immune response of yellowtail Seriola quinqueradiata to the bacterial fish pathogen Lactococcus garvieae.
复制标题
黄尾鰤对细菌性鱼类病原体加维乳球菌的保护性免疫反应。
DOI：
10.3354/dao037121
复制
发表时间：
1999
影响因子：
1.4
通讯作者：
T. Yoshida
中科院分区：
文献类型：
--
作者：
T. Ooyama;A. Kera;T. Okada;V. Inglis;T. Yoshida
用 2 种不同的加氏乳球菌菌苗、福尔马林灭活的 KG- 表型细胞（有荚膜表型）和福尔马林灭活的 KG+ 表型细胞（无荚膜表型）对黄尾鰤进行免疫。这两种注射疫苗为黄尾鱼提供了长期保护，使其免受封装的加维乳球菌菌株的人工感染，并对 KG+ 表型细胞具有持久的凝集滴度。然而，在给予每种菌苗的鱼中没有检测到针对 KG 表型细胞的凝集滴度或低凝集滴度。这些结果表明，KG-表型细胞中的荚膜明显影响其免疫原性，但赋予鱼类免受乳球菌感染保护的抗原可能位于KG+表型细胞的表面，而不是KG-表型细胞中的细胞荚膜。福尔马林灭活的 KG+ 表型细胞疫苗提供的保护似乎不具有毒株特异性。包膜的L. garvieae细胞被很好地吞噬，并且在用黄尾鱼免疫血清处理后，在KG-表型细胞中看到了菌毛样附属物。
Yellowtail Seriola quinqueradiata were immunized with 2 different Lactococcus garvieae bacterin, formalin-killed KG- phenotype cells (capsulated phenotype) and formalin-killed KG+ phenotype cells (unencapsulated phenotype). These 2 injected vaccines conferred long-term protection to yellowtail against an artificial infection of an encapsulated Lactococcus garvieae strain with long-lasting agglutinating titres against KG+ phenotype cells. However, no agglutinating titres or low agglutinating titres against KG- phenotype cells were detected in fish given each of these bacterin. These results suggested that a capsule in KG- phenotype cells apparently affects their immunogenicity, but the antigens which conferred protection to fish against lactococcal infection may be located on the surface of KG+ phenotype cells, and are not cell capsules in KG- phenotype cells. The protection offered by a formalin-killed KG+ phenotype cell vaccine would not appear to be strain specific. Encapsulated L. garvieae cells were well phagocytosed, and fimbrie-like appendages were seen in KG- phenotype cells after treatment with yellowtail immune serum.