EVIDENCE FOR A LOCUS ACTIVATION REGION - THE FORMATION OF DEVELOPMENTALLY STABLE HYPERSENSITIVE SITES IN GLOBIN-EXPRESSING HYBRIDS
EVIDENCE FOR A LOCUS ACTIVATION REGION - THE FORMATION OF DEVELOPMENTALLY STABLE HYPERSENSITIVE SITES IN GLOBIN-EXPRESSING HYBRIDS
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DOI：
10.1093/nar/15.24.10159
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发表时间：
1987-12-23
影响因子：
14.9
通讯作者：
GROUDINE, M
中科院分区：
文献类型：
--
作者：
FORRESTER, WC;TAKEGAWA, S;GROUDINE, M
我们分析了人非红系细胞与小鼠红白血病(MEL)细胞融合产生的体细胞杂交体细胞中人β-珠蛋白基因座的染色质结构。在这些杂交种中，人类成年的β-珠蛋白基因，但胚胎和胎儿的珠蛋白基因，都没有转录激活。此外，亲代非红系细胞(1，2)特有的DNaseI抗性β样珠蛋白基因座在大约80kb的区域内重组，包括激活的成人β-珠蛋白基因(2，3)的发育稳定的50kb5‘和20kb3’位点的形成。这些结果与发生在发育稳定的5‘和/或37’超敏位点上的事件对于β-珠蛋白基因座的激活是重要的，如果不是必要的，这一假说是一致的。
We have analyzed the chromatin structure of the human β - globin locus in somatic cell hybrids resulting from the fusion of human non-erythroid cells and mouse erythroleukemia (MEL) cells. In these hybrids, the human adult β-globin gene, but neither the embryonic nor fetal globin genes, is activated transcriptionally. In addition, the DNase I-resistant β-like globin locus characteristic of the parental non-erythroid human cells (1,2) is reorganized over an approximately 80 kb region, including the formation of the developmentally stable hypersensitive sites 50 kb 5′ and 20 kb 3′ of the activated adult β-globin gene (2,3). These results are consistent with the hypothesis that events occurring at the 5′ and/or 37′ developmentally stable hypersensitive sites are important, if not necessary, for the activation of the β-globin locus.