RRS1 and RPS4 provide a dual Resistance-gene system against fungal and bacterial pathogens
RRS1 and RPS4 provide a dual Resistance-gene system against fungal and bacterial pathogens
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DOI：
10.1111/j.1365-313x.2009.03949.x
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发表时间：
2009-10-01
期刊：
影响因子：
7.2
通讯作者：
Narusaka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Narusaka, Mari;Shirasu, Ken;Narusaka, Yoshihiro
胡氏炭疽菌是一种侵染十字花科植物的真菌病原菌，造成严重的农作物损失。我们利用图位克隆和自然变异分析的方法，对19个拟南芥生态型进行了抗性基因的定位。Higginsianum。该基因座命名为RCH 2（用于识别C. higginsianum）在拟南芥生态型Ws-0中广泛的抗病基因座簇MRC-J中作图。通过分析MRC-J区域内的自然变异，我们发现RRS 1（对青枯雷尔氏菌1的抗性）的等位基因含有可能影响编码蛋白的单核苷酸多态性。与这一发现相一致，通过筛选T-DNA标记的突变体库来鉴定两个敏感突变体，rrs 1 -1和rrs 1 -2，用于丧失对C的抗性。Higginsianum。筛选鉴定了在邻近基因RPS 4-Ws中具有5-bp缺失的另外的易感突变体（rps 4 -21），RPS 4-Ws是提供对假单胞菌烟草致病变种的抗性的充分表征的R基因。表达avrRps 4的番茄菌株DC 3000（Pst-avrRps 4）。rps 4 -21/rrs 1 -1双突变体对C. higginsianum作为单一突变体。我们还发现RRS 1和RPS 4都是抵抗R所必需的。青枯菌和Pst-avrRps 4.因此，RPS 4-Ws和RRS 1-Ws作为双重抗性基因系统发挥作用，防止三种不同病原体的感染。
Colletotrichum higginsianum is a fungal pathogen that infects a wide variety of cruciferous plants, causing important crop losses. We have used map-based cloning and natural variation analysis of 19 Arabidopsis ecotypes to identify a dominant resistance locus against C. higginsianum. This locus named RCH2 (for recognition of C. higginsianum) maps in an extensive cluster of disease-resistance loci known as MRC-J in the Arabidopsis ecotype Ws-0. By analyzing natural variations within the MRC-J region, we found that alleles of RRS1 (resistance to Ralstonia solanacearum 1) from susceptible ecotypes contain single nucleotide polymorphisms that may affect the encoded protein. Consistent with this finding, two susceptible mutants, rrs1-1 and rrs1-2, were identified by screening a T-DNA-tagged mutant library for the loss of resistance to C. higginsianum. The screening identified an additional susceptible mutant (rps4-21) that has a 5-bp deletion in the neighboring gene, RPS4-Ws, which is a well-characterized R gene that provides resistance to Pseudomonas syringae pv. tomato strain DC3000 expressing avrRps4 (Pst-avrRps4). The rps4-21/rrs1-1 double mutant exhibited similar levels of susceptibility to C. higginsianum as the single mutants. We also found that both RRS1 and RPS4 are required for resistance to R. solanacearum and Pst-avrRps4. Thus, RPS4-Ws and RRS1-Ws function as a dual resistance gene system that prevents infection by three distinct pathogens.