Monoclonal antibodies to surfactant proteins SP28-36 label canine type II and nonciliated bronchiolar cells by immunofluorescence.
Monoclonal antibodies to surfactant proteins SP28-36 label canine type II and nonciliated bronchiolar cells by immunofluorescence.
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表面活性剂蛋白 SP28-36 的单克隆抗体通过免疫荧光标记犬 II 型和非纤毛细支气管细胞。
DOI：
10.1164/ajrccm/137.2.399
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Benson,B
中科院分区：
文献类型：
--
作者：
Williams,MC;Hawgood,S;Schenk,DB;Lewicki,J;Phelps,MN;Benson,B
摘要 多种技术已表明肺表面活性剂的主要 28,000-至 36, OOO-道尔顿蛋白 (SP28-36) 是 II 型肺泡细胞的合成和分泌产物。这些细胞也分泌表面活性剂脂质。对人类和啮齿动物肺部的免疫细胞化学研究表明，小细支气管的无纤毛上皮细胞的合成细胞器和分泌颗粒中似乎含有 SP28-36。由于这些观察结果是使用针对 SP28-36 的多克隆抗体获得的，因此细支气管细胞染色可能是由于生化分析未检测到的污染抗体所致。为了阐明细支气管细胞在SP28-36代谢中的作用，我们制备了5种针对犬SP28-36的单克隆抗体。
SUMMARY The major 28,000-to 36, OOO-dalton proteins of pulmonary surfactant (SP28-36) have been shown by various techniques to be synthetic and secretory products of alveolar type II cells. Surfactant lipids are also secreted by these cells. Immunocytochemical studies of human and rodent lungs have indicated that nonciliated epithelial cells of small bronchioles appear to contain SP28-36 in their synthetic organelles and secretory granules. Because these observations were obtained with polyclonal antibodies against SP28-36, it was possible that bronchiolar cell staining was due to contaminant antibodies not detected by biochemical analyses. Toclarify the role of bronchiolar cells in the metabolism of SP28-36, we have prepared 5 monoclonal antibodies against canine SP28-36.