A simple hydroponic hardening system and the effect of nitrogen source on the acclimation of in vitro Cassava (Manihot esculenta Crantz)
A simple hydroponic hardening system and the effect of nitrogen source on the acclimation of in vitro Cassava (Manihot esculenta Crantz)
复制标题
简单水培硬化系统及氮源对离体木薯驯化的影响
DOI：
10.1007/s11627-016-9796-z
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Selvaraj MG.
中科院分区：
文献类型：
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作者：
Castnad O;Ogawa S;Medina A;Chavarriaga P;Selvaraj MG.
植物组织培养技术在木薯的大规模、快速、克隆增殖和遗传转化中得到广泛应用。该技术的主要局限性是脆弱的试管植物在繁殖或再生后的驯化期。当试管中的小植株被直接移到离体培养条件下时，大部分的离体培养植株的损失都发生了。本研究的目的是设计一种简单、快速、低维护的水培系统，通过对离体植物的快速驯化过程，提高离体条件下的移栽成活率。本研究建立了一个简单的水培系统，以加速木薯的驯化和增殖过程。该系统大大提高了试管和/或转基因株系的存活率，并减少了水培驯化繁殖所需的时间。为了评估其建立的幼苗驯化的有效性，我们分析了植物生长和田间存活率与响应不同的氮（N）源，使用不同的木薯种质。与单独的NH 4+或水处理相比，NO3−和NH 4 NO3的氮源增加了植物生长和根长。包括转基因株系在内的氮胁迫植株的温室和田间存活力在生长发育上存在显著差异。我们提出了一个简单的NO3−水培驯化系统，可以快速，廉价地建造和使用的木薯社区在世界各地。我们提出的N水培驯化系统的效率在转基因开发管道中得到验证，这将促进木薯分子育种。
Plant tissue culture technology is being widely used for large-scale, rapid, clonal multiplication and genetic transformation in cassava. The main limitation of this technology is the period of acclimation of the fragilein vitroplants after they have been multiplied or regenerated. Most losses ofin vitroplants occur when the plantlets are moved directly from the test tubes to the ex vitro conditions. Our aim was to design a simple, rapid, low-maintenance hydroponic system to improve survival rate of transplanting to the ex vitro conditions through the rapid acclimation process ofin vitroplants. In this paper, we have developed a simple hydroponic system to accelerate the cassava acclimation and multiplication process. This system considerably increased the survival percentage ofin vitroand/or transgenic lines and reduces the time requirement for multiplication by hydroponic acclimation. In order to assess the effectiveness of the acclimation of seedlings on their establishment, we analyzed plant growth and field survival rate with response to different nitrogen (N) sources using different cassava accessions. Nitrogen sources of NO3−and NH4NO3increased plant growth and root length compared to NH4+alone, or water treatments. The greenhouse and field survivability of N-hardened plants, including transgenic lines, were significantly different in growth and development. We present a simple NO3−hydroponic acclimation system that can be quickly and cheaply constructed and used by the cassava community around the world. The efficiency of our proposed N hydroponic acclimation system is validated in the transgenic development pipeline which will enhance the cassava molecular breeding.