Carbon dots-sensitized amorphous MoSx photoanode: Sequential electrodeposition preparation and dual amplified photoelectrochemical aptasensing of adenosine
Carbon dots-sensitized amorphous MoSx photoanode: Sequential electrodeposition preparation and dual amplified photoelectrochemical aptasensing of adenosine
复制标题
碳点敏化非晶MoS光阳极：腺苷的连续电沉积制备和双放大光电化学适体传感
DOI：
10.1016/j.bios.2019.111741
复制
发表时间：
2019-12-15
影响因子：
12.6
通讯作者：
Song, Wenbo
中科院分区：
文献类型：
--
作者：
Gao, Yao;Qi, Hui;Song, Wenbo
高可见光激活光电极的设计和制造对于精确检测生物系统中的生物分子至关重要。在此，基于碳点敏化非晶硫化钼（a-MoSx/CDs）光阳极和双放大策略，建立了一种用于特异性识别腺苷的超灵敏光电化学（PEC）适体传感器。通过顺序电沉积实现的异质结构光阳极显示出显着增强的光电流，在可见光照射下具有良好的稳定性和可重复性，这归功于高度激活的可见光吸收、均匀的覆盖和与底层基板的良好电接触，以及两个组件之间的能带对准。通过逐步固定互补DNA探针（NH2-DNA）和腺苷适体（Apt），然后亚甲蓝（MB）与Apt上的鸟嘌呤碱基结合，构建了用于腺苷检测的双放大自供电PEC适体传感器。基于CDs和MB的共敏效应，在0V（vs.SCE）下实现了0.01nM-1000nM浓度范围内腺苷的超灵敏、高亲和力测定，具有良好的稳定性和重现性。检测限低至 3.3 pM，其性能甚至超越了迄今为止报道的大多数传感器。这项工作强调了共敏化 a-MoS2 光阳极在超灵敏适体传感中的前景应用。
The design and fabrication of high visible-light activated photoelectrode are essential to precisely detect biomolecule in biological system. Herein, an ultrasensitive photoelectrochemical (PEC) aptasensor for specific recognition of adenosine is established based on carbon dots sensitized-amorphous molybdenum sulfide (a-MoSx/CDs) photoanode and dual amplification strategy. The heterostructured photoanode achieved by sequential electrodeposition reveals significantly boosted photocurrent with good stability and repeatability under visible light illumination, giving the credit to highly activated visible light absorption, uniform coverage and good electric contact to the underlying substrate, as well as the energy-band alignment between the two components. By stepwisely immobilizing complementary DNA probe (NH2-DNA) and adenosine aptamer (Apt), followed by methylene blue (MB) binding with the guanine base on Apt, a dual amplified self-powered PEC aptasensor for adenosine detection is constructed. Based on the co-sensitization effect of CDs and MB, ultrasensitive and high-affinitive determination of adenosine is realized over the concentration range of 0.01 nM-1000 nM at 0 V (vs. SCE), with satisfactory stability and reproducibility. The detection limit is as low as 3.3 pM, demonstrating a performance even surpassing most of the sensors reported so far. The prospective application of the co-sensitized a-MoS photoanode for ultrasensitive aptasensing is highlighted in this work.