The Effect of a Self-Assembling Peptide Nanofiber Scaffold (Peptide) When Used as a Wound Dressing for the Treatment of Deep Second Degree Burns in Rats
The Effect of a Self-Assembling Peptide Nanofiber Scaffold (Peptide) When Used as a Wound Dressing for the Treatment of Deep Second Degree Burns in Rats
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DOI：
10.1002/jbm.b.31226
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发表时间：
2009-05-01
影响因子：
3.4
通讯作者：
Zhao, Xiaojun
中科院分区：
文献类型：
--
作者：
Meng, Hui;Chen, Liyan;Zhao, Xiaojun
RADARADARARAADA（RADA 16-I）肽由16个交替的疏水性和亲水性（也交替的负电荷和正电荷）氨基酸组成，形成极其稳定的P-折叠片结构，然后自组装成纳米纤维，以产生高阶交织的可降解支架水凝胶。为了研究其治疗作用，用电烫伤机在雌性Sprague-Dawley大鼠背部皮肤造成部分厚度-深层皮肤损伤（深二度烧伤）模型。在形态学上仔细检查用RADA 16-I或对照材料处理的伤口，组织学和细胞水平。结果发现，RADA 16-I能使瘢痕形成时间和消失时间均比对照组（壳聚糖、聚（DL）-乳酸（PDLA）、I型胶原和空白）提前3-5天，创面收缩速度加快20-30%，无明显水肿。免疫组化结果显示，伤后用RADA 16-I处理创面，FGF和EGF在新生组织如表皮和腺体中均有明显表达。当肽原液从10 mg/mL稀释到0.17 mg/mL时，原子力显微镜（AFM）观察显示肽纳米纤维的形状从高度为4.8 ± 0.38 nm、宽度为61.6 ± 6.10 nm和长度为708 ± 80.2 nm的球形-碎片-簇状细丝改变，到具有1.4 +/- 0.36 nm、17.5 +/- 1.13 nm和1108 +/- 184 nm的一般细丝。表面孔隙率从49-70%逐渐降低到12- 28%。这些特征通过提供“理想敷料”湿润愈合微环境和三维支架有助于伤口愈合。这些结果表明，自组装肽可能是一个有前途的伤口敷料，简单，有效，负担得起的。(C)2008 Wiley Periodicals，Inc.生物医学材料研究杂志B部分：应用生物材料89 B：379-391，2009
RADARADARADARADA (RADA16-I) peptide, consisting of 16 alternating hydrophobic and hydrophilic (also alternating negative and positive charges) amino acids, forms extremely stable P-pleated sheet structure and then self-assembles into nanofibers to produce high-order interwoven nanofiber scaffold hydrogel. To investigate its therapeutic effects, a burn model of partial thickness-deep dermal injury (the deep second degree burns) was performed at the dorsal skin of female Sprague-Dawley rats with an electrical scalding machine. The wounds treated with either RADA16-I or control materials were carefully examined at morphological., histological and cellular levels. We found that RADA16-I can advance the time of eschar appearance and the time of eschar disappearance both by 3-5 days, and speed up wound contraction by 20-30% compared with contrast groups (chitosan, poly(DL)-lactic acid (PDLA), collagen I and the blank) without obvious edema. Immunohistochemical studies showed that both FGF and EGF were obviously expressed in nascent tissue such as epidermis and glands when wounds were treated with the RADA16-I after injury. When peptide stock solution was diluted from 10 to 0.17 mg/mL, atomic force microscopy (AFM) observation showed that the shape of peptide nanofibers changed from the globular-pieces-clustered filaments with 4.8 +/- 0.38 nm in height, 61.6 +/- 6.10 nm in width and 708 +/- 80.2 nm in length, to general filaments with 1.4 +/- 0.36 nm, 17.5 +/- 1.13 nm and 1108 +/- 184 nm. The nanofiber surface porosity gradually decreased from 49-70% to 12-28%. These characteristics contribute to wound healing by offering an "ideal dressing" moist healing microenvironment and a nanofiber 3D scaffold. These results suggest that the self-assembling peptide might be a promising wound dressing with being simple, effective, and affordable. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 89B: 379-391, 2009