Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA.
Trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid RNA.
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DOI：
10.1093/nar/gkq1051
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发表时间：
2011-03
影响因子：
14.9
通讯作者：
Gago S
中科院分区：
文献类型：
--
作者：
Carbonell A;Flores R;Gago S
具有不连续或延伸茎I和具有三级稳定基序（TSM）的反式切割锤头先前已在低Mg 2+浓度下针对短RNA底物在体外进行了测试。然而，这些核酶在体外和体内靶向更长和结构化RNA的潜力尚未被研究。在这里，我们报告了在体外切割短RNA和464-nt的高度结构化的RNA从马铃薯纺锤块茎类病毒（PSTVd）锤头与不连续和扩展格式在亚毫摩尔Mg 2+。在此条件下，茄潜类病毒和桃潜花叶类病毒（PLMVd）分别以不连续型和扩展型的形式表现出最强的活性。此外，具有天然TSM的PLMVd衍生的锤头状蛋白在体内对相同的长底物表现出活性，并干扰系统性PSTVd感染，从而加强了这类核酶具有控制致病性RNA复制子的潜力的想法。
Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the potential of these ribozymes for targeting longer and structured RNAs in vitro and in vivo has not been examined. Here, we report the in vitro cleavage of short RNAs and of a 464-nt highly structured RNA from potato spindle tuber viroid (PSTVd) by hammerheads with discontinuous and extended formats at submillimolar Mg2+. Under these conditions, hammerheads derived from eggplant latent viroid and peach latent mosaic viroid (PLMVd) with discontinuous and extended formats, respectively, where the most active. Furthermore, a PLMVd-derived hammerhead with natural TSMs showed activity in vivo against the same long substrate and interfered with systemic PSTVd infection, thus reinforcing the idea that this class of ribozymes has potential to control pathogenic RNA replicons.
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影响因子：
3.9
作者：
Castanotto, D;Li, JR;Rossi, JJ
通讯作者：
Rossi, JJ
DOI：
10.1073/pnas.89.9.3711
发表时间：
1992-05-01
影响因子：
11.1
作者：
HERNANDEZ, C;FLORES, R
通讯作者：
FLORES, R
DOI：
10.1073/pnas.94.21.11262
发表时间：
1997-10-14
影响因子：
11.1
作者：
Navarro, B;Flores, R
通讯作者：
Flores, R
DOI：
10.1073/pnas.85.23.9128
发表时间：
1988-12-01
影响因子：
11.1
作者：
BRANCH, AD;BENENFELD, BJ;ROBERTSON, HD
通讯作者：
ROBERTSON, HD
在自切除位点之前的三核苷酸对茄子潜伏病毒锤的影响：转录后和后的自结论的差异可能解释了大多数天然锤头中缺乏三核苷酸AUC的差异。
影响因子：
14.9
作者：
Carbonell, Alberto;De la Pena, Marcos;Flores, Ricardo;Gago, Selma
通讯作者：
Gago, Selma