Sampling for the incidence of aphid-transmitted viruses in snap bean
Sampling for the incidence of aphid-transmitted viruses in snap bean
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DOI：
10.1094/phyto-95-1405
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发表时间：
2005-12-01
期刊：
影响因子：
3.2
通讯作者：
Nault, BA
中科院分区：
文献类型：
--
作者：
Shah, DA;Dillard, HR;Nault, BA
在2002年和2003年收集的数据，在纽约州的商业油豆角领域的Alcoholmosaic病毒和黄瓜花叶病毒的感染发病率被用来开发疾病发病率（p（低））和样本量之间的关系，同时占固有的空间聚集观察到这两种病毒感染的植物。对于由300个取样植物组成的计划（N = 60个样方，n = 5个植物/样方），从阳性组的发生率（p（高）;测试N = 60个分组样品）估计p（低）提供与单独测试200个植物相同的p（低）精度，高达约p（低）= 0.2。在此之上，通过组检验获得的p（低）的置信区间宽度明显大于通过检验个体植物获得的宽度。我们的研究结果建议使用分组测试，直到phigh在[0.35，0.59]范围内，这对应于p（低）在[0.1，0.2]。结果表明，组测试可以更经济的比单个植物的测试没有损失的精度，在较低的感染率。提出的方法提供了一个总体框架，抽样和估计发病率的其他蚜虫传播的病毒在菜豆。
Data collected in 2002 and 2003 on Alfalfa mosaic virus and Cucumber mosaic virus incidences of infection in commercial snap bean fields in New York State were used to develop relationships between disease incidence (p(low)) and sample size while accounting for the inherent spatial aggregation of infected plants observed with these two viruses. For a plan consisting of 300 sampled plants (N = 60 quadrats, n = 5 plants per quadrat), estimating p(low) from the incidence of positive groups (p(high); testing of N = 60 grouped samples) provides the same precision in p(low) as testing 200 plants individually, up to about p(low) = 0.2. Above that, the confidence interval width for p(low) obtained via group testing becomes markedly larger than the width obtained by testing individual plants. Our results suggest using group testing until phigh is in the range [0.35, 0.59], which corresponds to p(low) in [0.1, 0.2]. Results indicate that group testing can be more economical than the testing of individual plants without loss of precision, at lower incidences of infection. The approach presented provides a general framework for sampling and the estimation of incidence of other aphid-transmitted viruses in snap bean.