Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property
Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property
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DOI：
10.1016/j.bbrc.2007.05.212
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发表时间：
2007-08-10
影响因子：
3.1
通讯作者：
Dezawa, Mari
中科院分区：
文献类型：
--
作者：
Shimizu, Satoshi;Kitada, Masaaki;Dezawa, Mari
我们研究了人骨髓基质细胞(MSCs)作为神经损伤移植治疗来源的可行性。人骨髓间充质干细胞经过一系列含有还原剂、维甲酸和多种营养因子的处理。形态和免疫细胞化学显示，经处理的细胞在体外分化为雪旺细胞特征。将细胞填充到可渗透管中，移植到大鼠坐骨神经缝隙中，在免疫抑制剂的控制下随访3周。与未经处理的人MSCs相比，分化后的人MSCs在体内表达雪旺细胞标志，并支持再生轴突。这些结果表明，人骨髓间充质干细胞可以被诱导为雪旺细胞的替代物，有可能应用于神经再生。(C)2007 Elsevier Inc.保留所有权利。
We examined the availability of human bone marrow stromal cells (MSCs) as a source of transplantation therapy in nerve injury. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid and a combination of trophic factors. Morphologically and immunocytochemically, such treated cells differentiated into Schwann cell characteristics in vitro. Cells were filled into a transpermeable tube, transplanted into the gap made in the rat sciatic nerve of a rat and followed up to 3 weeks under the control of immunosuppressant. In contrast to untreated human MSCs, differentiated human MSCs expressed Schwann cell markers in vivo and supported regenerating axons. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration. (c) 2007 Elsevier Inc. All rights reserved.