Polyclonal and antigen-specific responses of T cells and T cell subsets.
Polyclonal and antigen-specific responses of T cells and T cell subsets.
复制标题
T 细胞和 T 细胞亚群的多克隆和抗原特异性反应。
DOI：
10.1007/978-1-59745-242-7_19
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Cook,RobertT
中科院分区：
文献类型：
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作者：
Young,BettyM;Wiechert,Susan;Coleman,RuthA;Gurung,Prajwal;Cook,RobertT
评估T细胞的功能反应对于确定慢性酒精滥用和其他导致免疫功能障碍的疾病引起的免疫缺陷的机制(S)具有重要意义。长期暴露在水中20%(w/v)乙醇的小鼠出现免疫缺陷，T细胞活化异常，总数减少，CD4/CD8比率增加，记忆/幼稚表型比率增加。这些细胞在用模型感染性生物接种乙醇小鼠后也有异常的抗原特异性反应。对这些细胞功能异常的研究需要一个可靠的系统，该系统能够在体外提供适当的激活刺激，以在浓缩或纯化的T细胞中产生多克隆或抗原特异性反应，而不受先前乙醇暴露的辅助细胞的影响。在本章中，我们描述了通过T细胞受体来评估T细胞对多克隆刺激的反应的方案，并使用了一种模型传染病细菌，单核细胞增生性李斯特菌，它允许在先前接种后评估T细胞对细菌的特定多肽表位的反应。
Evaluation of the functional responses of T cells is of importance in determining the mechanism(s) of immunodeficiency resulting from chronic alcohol abuse and other conditions that lead to immune dysfunction. Mice that are chronically exposed to 20% (w/v) ethanol in water develop immunodeficiency and have T cells with abnormal activation profiles, reduced total numbers, increased CD4/CD8 ratios, and an increased memory/naïve phenotype ratio. These cells also have abnormal antigen-specific responses after inoculation of the ethanol mice with model infectious organisms. Study of the functional abnormalities of these cells requires a reliable system that can present appropriate activation stimuli in vitro for the generation of polyclonal or antigen-specific responses in enriched or purified T cells, free of the influence of previously ethanol exposed accessory cells. In this chapter, we describe protocols to assess the T cell response to polyclonal stimulation through the T cell receptor and the use of a model infectious disease bacterium,Listeria monocytogenes, that allows evaluation of the T-cell response to specific peptide epitopes of the bacterium after previous inoculation.