Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats
Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats
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DOI：
10.1007/s00418-006-0171-8
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发表时间：
2006-09-01
影响因子：
2.3
通讯作者：
Mitani, Hideo
中科院分区：
文献类型：
--
作者：
Mitani, Hidetoshi;Takahashi, Ichiro;Mitani, Hideo
一种带有血栓反应蛋白基序的去整合素和金属蛋白酶(adamalysin-thetbospondins，ADAMTS)可降解软骨中主要的细胞外基质(ECM)成分--聚集素。下颌突软骨不同于原始软骨，如关节软骨和生长板软骨，其细胞外基质的代谢、增殖和分化。下颌骨髁状突软骨在生长期既是关节软骨又是生长板软骨，生长后仍是关节软骨。我们推测，在生长和衰老过程中，髁突和原始软骨之间的功能和ECM差异会导致aggrecan和ADAMTS-1、-4和-5基因表达模式和水平的差异。我们用原位杂交和半定量RT-PCR方法检测了这些分子在生长和老化过程中在髁突软骨和原代软骨中的表达。在本研究中测试的软骨中，所有的ADAMTS都呈现出特征性的、年龄相关的表达模式和水平。ADAMTS-5在生长过程中主要参与生长板和髁突软骨细胞外基质的代谢。ADAMTS-1和ADAMTS-4可能参与了关节软骨细胞外基质的翻转。本研究结果显示，原、次级软骨细胞外基质的代谢及相关蛋白水解酶的表达在生长和老化过程中可能存在差异调节。
A disintegrin and metalloproteinase with thrombospondin motif (adamalysin-thrombospondins, ADAMTS) degrades aggrecan, one of the major extracellular matrix (ECM) components in cartilage. Mandibular condylar cartilage differs from primary cartilage, such as articular and growth plate cartilage, in its metabolism of ECM, proliferation, and differentiation. Mandibular condylar cartilage acts as both articular and growth plate cartilage in the growing period, while it remains as articular cartilage after growth. We hypothesized that functional and ECM differences between condylar and primary cartilages give rise to differences in gene expression patterns and levels of aggrecan and ADAMTS-1, -4, and -5 during growth and aging. We employed in situ hybridization and semiquantitative RT-PCR to identify mRNA expression for these molecules in condylar cartilage and primary cartilages during growth and aging. All of the ADAMTSs presented characteristic, age-dependent expression patterns and levels among the cartilages tested in this study. ADAMTS-5 mainly contributed to ECM metabolism in growth plate and condylar cartilage during growth. ADAMTS-1 and ADAMTS-4 may be involved in ECM turn over in articular cartilage. The results of the present study reveal that ECM metabolism and expression of related proteolytic enzymes in primary and secondary cartilages may be differentially regulated during growth and aging.