MN/CA9:: a potential gene marker for detection of malignant cells in effusions
MN/CA9:: a potential gene marker for detection of malignant cells in effusions
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DOI：
10.1080/13547500601068192
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发表时间：
2007-03-01
期刊：
影响因子：
2.6
通讯作者：
Genin, C.
中科院分区：
文献类型：
--
作者：
Li, G.;Passebosc-Faure, K.;Genin, C.
许多癌症会导致恶性积液。恶性细胞在胸腔积液中的存在对诊断、肿瘤分期和预后有重要意义。恶性细胞的检测目前对细胞病理学家来说是一个挑战。需要新的辅助方法。虽然渗出液为分子检测提供了很好的材料，但可用的分子标志物极其有限，这阻碍了其临床应用。MN/CA9在肺癌、乳腺癌、结肠癌、肾癌等多种肿瘤中是一种有价值的标志物。本研究旨在探讨MN/CA9作为一种新的检测胸腔积液癌细胞的分子标志物。采用逆转录聚合酶链式反应(RT-PCR)检测71例胸腔积液中MN/CA9基因的表达，其中癌性胸水59例，良性胸腔积液12例。乳腺癌15/16、肺癌10/11、卵巢癌4/4、结直肠癌2/3、不明部位癌5/6、间皮瘤7/8、其他癌10/11例胸腔积液中有53例(89.8%)MN/CA9基因表达。此外，MN/CA9在18例肿瘤细胞学阴性患者中有13例阳性(72.2%)。对照组仅1/12(8.3%)的胸腔积液中检出MN/CA9(p<；0.01)。MN/CA9基因表达的敏感性和特异性分别为89.8%和91.7%。我们的初步结果表明，MN/CA9可能是一种检测渗出液中恶性细胞的潜在标志物。需要一项大规模的研究来证实这些结果。
Many cancers cause malignant effusions. The presence of malignant cells in effusions has implications in diagnosis, tumour staging and prognosis. The detection of malignant cells currently presents a challenge for cytopathologists. New adjunctive methods are needed. Although the effusions provide excellent materials for molecular assay, the available molecular markers are extremely limited, which hinders its clinical application. MN/CA9 has proved to be a valuable marker in many cancers such as lung, breast, colon, kidney, etc. The present study was to evaluate MN/CA9 as a new molecular marker for the detection of cancer cells in pleural effusions. Seventy-one pleural effusions including 59 malignant effusions from patients with cancer, and 12 patients with benign diseases as a control, were subjected to RT-PCR for detection of MN/CA9 gene expression. MN/CA9 gene expression was detected in 53/59 (89.8%) pleural effusions from cancer patients (15/16 for breast cancers, 10/11 for lung cancers, 4/4 for ovary cancers, 2/3 for colon-rectal cancers, 5/6 for cancers of unknown site, 7/8 for mesothelioma and 10/11 for other cancers). Furthermore, MN/CA9 was positive in 13/18 (72.2%) of cytologically negative effilsions of cancer patients. MN/CA9 was detected in only 1/12 (8.3%) effusions from the control patients (p < 0.01). The sensitivity and specificity of MN/CA9 gene expression were, respectively, 89.8% and 91.7%. Our preliminary results suggest that MN/CA9 could be a potential marker for the detection of malignant cells in effilsions. A large-scale study is needed to confirm these results.