Antagonistic Interaction between Auxin and SA Signaling Pathways Regulates Bacterial Infection through Lateral Root in Arabidopsis
Antagonistic Interaction between Auxin and SA Signaling Pathways Regulates Bacterial Infection through Lateral Root in Arabidopsis
复制标题
生长素和 SA 信号通路之间的拮抗相互作用通过拟南芥侧根调节细菌感染
DOI：
10.1016/j.celrep.2020.108060
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发表时间：
2020-08-25
期刊：
影响因子：
8.8
通讯作者：
Ding, Zhaojun
中科院分区：
文献类型：
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作者：
Kong, Xiangpei;Zhang, Chunlei;Ding, Zhaojun
病原体进入宿主组织是感染的关键和第一步。在植物中，侧根(LRs)是病原体的潜在进入和定植场所。在这里，使用绿色荧光蛋白标记的致病细菌丁香假单胞菌。以番茄品系DC3000(PTO DC3000)为材料，我们观察到Pto DC3000通过在拟南芥中出现的LRs入侵植物。PTO DC3000强烈诱导LR的形成，这一过程依赖于生长素反应因子7(ARF7)/ARF19-器官侧边域(LBD)调节模块。我们发现水杨酸(SA)抑制LR的形成，几个SA信号缺陷的突变体也参与了PTO DC3000诱导的LR的形成。值得注意的是，ARF7是一种已知的LR发育的正向调节因子，它直接抑制PR1和PR2的转录，促进LR的发育。本研究表明，ARF7介导的生长素信号与SA信号相互拮抗，通过调节LR的发育来控制细菌感染。
Pathogen entry into host tissues is a critical and first step in infections. In plants, the lateral roots (LRs) are a potential entry and colonization site for pathogens. Here, using a GFP-labeled pathogenic bacterium Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), we observe that virulent Pto DC3000 invades plants through emerged LRs in Arabidopsis. Pto DC3000 strongly induced LR formation, a process that was dependent on the AUXIN RESPONSE FACTOR7 (ARF7)/ARF19-LATERAL ORGAN BOUNDARIES-DOMAIN (LBD) regulatory module. We show that salicylic acid (SA) represses LR formation, and several mutants defective in SA signaling are also involved in Pto DC3000-induced LR development. Significantly, ARF7, a well-documented positive regulator of LR development, directly represses the transcription of PR1 and PR2 to promote LR development. This study indicates that ARF7-mediated auxin signaling antagonizes with SA signaling to control bacterial infection through the regulation of LR development.